4.7 Article

Recombinase polymerase amplification in combination with electrochemical readout for sensitive and specific detection of PIK3CA point mutations

Related references

Note: Only part of the references are listed.
Review Biochemical Research Methods

Electrochemical biosensors for analysis of DNA point mutations in cancer research

Katerina Ondraskova et al.

Summary: This review highlights the importance of DNA point mutations in cancer and discusses commonly used screening methods. It also explores the advantages and challenges of electrochemical biosensors as a potential alternative technology.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2023)

Article Biochemistry & Molecular Biology

Combining recombinase polymerase amplification and DNA-templated reaction for SARS-CoV-2 sensing with dual fluorescence and lateral flow assay output

Lluc Farrera-Soler et al.

Summary: The early phase of the SARS-CoV-2 pandemic posed a significant diagnostic challenge. In this study, a method combining recombinase polymerase amplification and a DNA-templated reaction was developed for dual readout detection of the virus, using either fluorescence or naked eye detection. This method offers a potential solution for rapid and accurate detection of SARS-CoV-2.

BIOPOLYMERS (2022)

Article Agronomy

A Label-Free Electrochemical Impedance Genosensor Coupled with Recombinase Polymerase Amplification for Genetically Modified Maize Detection

Dandan Cui et al.

Summary: An unmarked electrochemical impedance genosensor using gold carbon dots has been developed for the detection of genetically modified crops. This sensor is portable and easy to use, making it suitable for resource-limited laboratories.

AGRICULTURE-BASEL (2022)

Article Chemistry, Analytical

A general RPA-CRISPR/Cas12a sensing platform for Brucella spp. detection in blood and milk samples

Jianhao Xu et al.

Summary: Brucellosis, caused by Brucella spp., is a disease that leads to long-term damage in humans and livestock. Researchers have developed a dual biosensor based on CRISPR/Cas12a, which can rapidly and accurately detect various Brucella strains and distinguish them from non-target bacteria.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

Article Pathology

Characterizing the Impact of Primer-Template Mismatches on Recombinase Polymerase Amplification

Matthew Higgins et al.

Summary: This study systematically characterizes the impact of mismatches on the recombinase polymerase amplification (RPA) reaction when located in the 30-anchor region of the primer-template complex. The study identifies the nucleotides involved and the position of each mismatch as factors influencing the size of the impact, with terminal cytosine-thymine and guanine-adenine mismatches being the most detrimental.

JOURNAL OF MOLECULAR DIAGNOSTICS (2022)

Article Chemistry, Analytical

Detection of breast cancer-related point-mutations using screen-printed and gold-plated electrochemical sensor arrays suitable for point-of-care applications

Vanessa Thoeny et al.

Summary: Three significant PIK3CA point-mutations (H1047R, E545K, and E542K) in breast cancer were detected using a hybridization-based microarray assay. Optimal conditions were achieved and a fluorescence-based microarray assay was developed for simultaneous and specific detection. An enzyme-amplified electrochemical assay on a screen-printed sensor array allowed for the detection of PIK3CA point-mutations with a detection limit of 10 pM.

TALANTA OPEN (2022)

Article Biophysics

Electric field-enhanced electrochemical CRISPR biosensor for DNA detection

Ziyue Li et al.

Summary: The developed EFE electrochemical CRISPR biosensor is a simple, versatile tool for detecting DNA targets in a homogeneous solution phase, achieving sensitive detection without the need for complicated immobilization processing. By leveraging electric field enhancement and diffusivity differences, the sensor provides a robust and sensitive method for nucleic acid-based molecular diagnostics.

BIOSENSORS & BIOELECTRONICS (2021)

Article Chemistry, Analytical

Allele-Specific Recombinase Polymerase Amplification to Detect Sickle Cell Disease in Low-Resource Settings

Mary E. Natoli et al.

Summary: A rapid and low-cost nucleic acid test for detecting the point mutation in SCD has been developed in this study, showing high sensitivity and specificity with a turnaround time of less than 30 minutes. The test can be performed near the point of care and may be adapted for detecting other disease-causing point mutations in genomic DNA.

ANALYTICAL CHEMISTRY (2021)

Article Engineering, Biomedical

Woven Electroanalytical Biosensor for Nucleic Acid Amplification Tests

Shirin Khaliliazar et al.

Summary: Fiber-based biosensors with microfluidic structures and electroanalytical readout are used for Nucleic Acid Amplification Testing. Two types of fiber-based electrodes are characterized, and a woven electrochemical DNA sensor is fabricated for specific detection of genomic DNA of Staphylococcus epidermidis.

ADVANCED HEALTHCARE MATERIALS (2021)

Article Chemistry, Analytical

A polyA DNA probe-based ultra-sensitive and structure-distinguishable electrochemical biosensor for the analysis of RNAi transgenic maize

Li Xu et al.

Summary: An electrochemical biosensor for transgene-derived long RNA was developed using a poly-adenine DNA capture probe, with enhanced stability and optimized surface density for hybridization. Multiple reporter probe system and RT-RPA technology improved sensitivity and selectivity for RNAi GMO samples, showing great potential in detection and analysis of functional RNA molecules.

ANALYST (2021)

Article Chemistry, Analytical

Yersinia pestis detection using biotinylated dNTPs for signal enhancement in lateral flow assays

Salah Kortli et al.

ANALYTICA CHIMICA ACTA (2020)

Article Oncology

Frequency and spectrum of PIK3CA somatic mutations in breast cancer

Olga Martinez-Saez et al.

BREAST CANCER RESEARCH (2020)

Article Medical Laboratory Technology

Comparison of BEAMing and Droplet Digital PCR for Circulating Tumor DNA Analysis

Ben O'Leary et al.

CLINICAL CHEMISTRY (2019)

Article Biochemical Research Methods

Blocked recombinase polymerase amplification for mutation analysis of PIK3CA gene

Sara Martorell et al.

ANALYTICAL BIOCHEMISTRY (2018)

Article Chemistry, Multidisciplinary

Combinatorial Probes for High-Throughput Electrochemical Analysis of Circulating Nucleic Acids in Clinical Samples

Jagotamoy Das et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Review Chemistry, Analytical

Recombinase polymerase amplification: Basics, applications and recent advances

Ivan Magrina Lobato et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2018)

Article Cell Biology

Enhanced detection of circulating tumor DNA by fragment size analysis

Florent Mouliere et al.

SCIENCE TRANSLATIONAL MEDICINE (2018)

Review Electrochemistry

Electrochemical Biosensors in Point-of-Care Devices: Recent Advances and Future Trends

Everson T. S. G. da Silva et al.

CHEMELECTROCHEM (2017)

Review Medical Laboratory Technology

Recombinase Polymerase Amplification for Diagnostic Applications

Rana K. Daher et al.

CLINICAL CHEMISTRY (2016)

Article Genetics & Heredity

Fragment Length of Circulating Tumor DNA

Hunter R. Underhill et al.

PLOS GENETICS (2016)

Review Microbiology

Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects

Tsugunori Notomi et al.

JOURNAL OF MICROBIOLOGY (2015)

Article Chemistry, Multidisciplinary

An electrochemical clamp assay for direct, rapid analysis of circulating nucleic acids in serum

Jagotamoy Das et al.

NATURE CHEMISTRY (2015)

Review Medical Laboratory Technology

Circulating Tumor DNA as a Liquid Biopsy for Cancer

Ellen Heitzer et al.

CLINICAL CHEMISTRY (2015)

Article Oncology

Detection of Cancer DNA in Plasma of Patients with Early-Stage Breast Cancer

Julia A. Beaver et al.

CLINICAL CANCER RESEARCH (2014)

Review Chemistry, Analytical

Screen-printed electrodes for biosensing: a review (2008-2013)

Zahra Taleat et al.

MICROCHIMICA ACTA (2014)

Article Cell Biology

Detection of Circulating Tumor DNA in Early- and Late-Stage Human Malignancies

Chetan Bettegowda et al.

SCIENCE TRANSLATIONAL MEDICINE (2014)

Article Biochemical Research Methods

Comparison of different methods for generation of single-stranded DNA for SELEX processes

M. Svobodova et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2012)

Article Biochemical Research Methods

Evaluation of techniques for generation of single-stranded DNA for quantitative detection

Laia Civit et al.

ANALYTICAL BIOCHEMISTRY (2012)

Review Chemistry, Multidisciplinary

Electrochemistry of Nucleic Acids

Emil Palecek et al.

CHEMICAL REVIEWS (2012)

Article Chemistry, Multidisciplinary

DNA Electrochemistry with Tethered Methylene Blue

Catrina G. Pheeney et al.

LANGMUIR (2012)

Review Chemistry, Multidisciplinary

Electrochemical biosensors

Niina J. Ronkainen et al.

CHEMICAL SOCIETY REVIEWS (2010)

Review Chemistry, Multidisciplinary

Self-assembled monolayers of thiols and dithiols on gold: new challenges for a well-known system

C. Vericat et al.

CHEMICAL SOCIETY REVIEWS (2010)

Article Chemistry, Multidisciplinary

Electrochemical nucleic acid-based biosensors: Concepts, terms, and methodology (IUPAC Technical Report)

Jan Labuda et al.

PURE AND APPLIED CHEMISTRY (2010)

Review Biochemistry & Molecular Biology

Isothermal DNA amplification in vitro: the helicase-dependent amplification system

Yong-Joo Jeong et al.

CELLULAR AND MOLECULAR LIFE SCIENCES (2009)

Article Chemistry, Multidisciplinary

An Electrochemical Sensor for Single Nucleotide Polymorphism Detection in Serum Based on a Triple-Stem DNA Probe

Yi Xiao et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2009)

Article Biochemistry & Molecular Biology

Predicting stability of DNA duplexes in solutions containing magnesium and monovalent cations

Richard Owczarzy et al.

BIOCHEMISTRY (2008)

Article Electrochemistry

Electrochemical characterization of different screen-printed gold electrodes

Raquel Garcia-Gonzalez et al.

ELECTROCHIMICA ACTA (2008)

Article Biophysics

DNA single-base mismatch study with an electrochemical enzymatic genosensor

Patricia Abad-Valle et al.

BIOSENSORS & BIOELECTRONICS (2007)

Article Biochemistry & Molecular Biology

DNA detection using recombination proteins

Olaf Piepenburg et al.

PLOS BIOLOGY (2006)

Article Biochemistry & Molecular Biology

Echinomycin and cobalt-phenanthroline as redox indicators of DNA hybridization at gold electrodes

H Karadeniz et al.

FRONTIERS IN BIOSCIENCE-LANDMARK (2006)

Article Chemistry, Analytical

Charge transfer through DNA: A selective electrochemical DNA biosensor

ELS Wong et al.

ANALYTICAL CHEMISTRY (2006)

Review Chemistry, Multidisciplinary

Self-assembled monolayers of thiolates on metals as a form of nanotechnology

JC Love et al.

CHEMICAL REVIEWS (2005)

Article Multidisciplinary Sciences

High frequency of mutations of the PIK3CA gene in human cancers

Y Samuels et al.

SCIENCE (2004)

Article Biochemistry & Molecular Biology

Helicase-dependent isothermal DNA amplification

M Vincent et al.

EMBO REPORTS (2004)

Review Oncology

The phosphatidylinositol 3-kinase-AKT pathway in human cancer

I Vivanco et al.

NATURE REVIEWS CANCER (2002)

Article Biotechnology & Applied Microbiology

Mutation detection by electrocatalysis at DNA-modified electrodes

EM Boon et al.

NATURE BIOTECHNOLOGY (2000)

Article Biochemistry & Molecular Biology

Loop-mediated isothermal amplification of DNA

Tsugunori Notomi et al.

NUCLEIC ACIDS RESEARCH (2000)