Inducible nitric oxide synthase activity mediates TNF-a-induced endothelial cell dysfunction

Inducible nitric oxide synthase (iNOS) and vascular endothelial dysfunction have been implicated in the development and progression of atherosclerosis. This study aimed to elucidate the role of iNOS in vascular endothelial dysfunction. Ultrahigh performance liquid chromatography-quadrupole time-of-flight mass spectrometry combined with multivariate data analysis was used to characterize the metabolic changes in human umbilical vein endothelial cells (HUVECs) in response to different treatment conditions. In addition, molecular biology techniques were employed to explain the molecular mechanisms underlying the role of iNOS in vascular endothelial dysfunction. Tumor necrosis factor-a (TNF-a) enhances the expression of iNOS, TXNIP, and the level of reactive oxygen species (ROS) facilitates the entry of nuclear factor-?B (NF-?B) into the nucleus and promotes injury in HUVECs. iNOS deficiency reversed the TNF-a-mediated pathological changes in HUVECs. Moreover, TNF-a increased the expression of tumor necrosis factor receptor-2 (TNFR-2) and the levels of p-I?Ba and IL-6 proteins and CD31, ICAM-1, and VCAM-1 protein expression, which was significantly reduced in HUVECs with iNOS deficiency. In addition, treating HUVECs in the absence or presence of TNF-a or iNOS, respectively, enabled the identification of putative endogenous biomarkers associated with endothelial dysfunction. These biomarkers were involved in critical metabolic pathways, including glycosylphosphatidylinositol-anchor biosynthesis, amino acid metabolism, sphingolipid metabolism, and fatty acid metabolism. iNOS deficiency during vascular endothelial dysfunction may affect the expression of TNFR-2, vascular adhesion factors, and the level of ROS via cellular metabolic changes, thereby attenuating vascular endothelial dysfunction.

Automated summary

This study elucidates the role of iNOS in vascular endothelial dysfunction through cellular experiments and metabolic analysis. The findings suggest that iNOS deficiency can reverse the pathological changes induced by TNF-α and alleviate vascular endothelial dysfunction.