Derivation of trophoblast stem cells from human expanded potential stem cells

Human trophoblast development study has long been limited by the lack of suitable materials. Here we present a detailed protocol for the differentiation of human expanded potential stem cells (hEPSCs) into human trophoblast stem cells (TSCs) and for the subsequent establishment of TSC lines. The hEPSC-derived TSC lines can be continuously passaged and are functional in further differentiation into syncytiotrophoblasts and extravillous trophoblasts. The hEPSC-TSC system offers a valuable cell source for studying human trophoblast development in pregnancy. For complete details on the use and execution of this protocol, please refer to Gao et al. (2019)1 and Ruan et al. (2022).2

Automated summary

This article presents a detailed protocol for differentiating human expanded potential stem cells (hEPSCs) into human trophoblast stem cells (TSCs) and establishing TSC lines. The hEPSC-derived TSC lines can be continuously passaged and are capable of further differentiation into syncytiotrophoblasts and extravillous trophoblasts. The hEPSC-TSC system provides a valuable cell source for studying human trophoblast development in pregnancy.