Protocol for colitis-associated colorectal cancer murine model induced by AOM and DSS

Inflammatory bowel diseases (IBDs) contribute to the tumorigenesis of colorectal cancer (CRC). Here, we describe a step-by-step protocol for the construction of colitis-associated CRC murine model by sequential utilization of azoxymethane and dextran sulfate sodium. We also detail steps to determine the degree of murine intestinal inflammation and to generate colorectum Swiss roll for further histopathological analyses. This is a convenient and reproducible protocol for colitis-associated CRC murine model by the induction of general chemical reagents.For complete details on the use and execution of this protocol, please refer to Yang et al. (2022).(1)

Automated summary

This article describes a step-by-step protocol for constructing a murine model of colitis-associated colorectal cancer (CRC) using azoxymethane and dextran sulfate sodium. It also provides detailed steps for assessing the degree of intestinal inflammation and generating colorectum Swiss roll for histopathological analyses. This protocol offers a convenient and reproducible method for inducing colitis-associated CRC in mice using general chemical reagents.