Analytical and clinical performances of seven direct detection assays for SARS-CoV-2

Article Virology

Development of a point-of-care test to detect SARS-CoV-2 from saliva which combines a simple RNA extraction method with colorimetric reverse transcription loop-mediated isothermal amplification detection

Wataru Yamazaki et al.

Summary: The study has successfully developed a simple and rapid method for extracting RNA from saliva and conducting COVID-19 testing, which can be used in field settings to potentially reduce the risk of infection.

JOURNAL OF CLINICAL VIROLOGY (2021)

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Article Infectious Diseases

Evaluation of clinical utility of novel coronavirus antigen detection reagent, Espline (R) SARS-CoV-2

Kotaro Aoki et al.

Summary: The study evaluated the sensitivity of the SARS-CoV-2 antigen test Espline (R) compared to the reference test RT-PCR, showing that the antigen test can be helpful in preventing virus transmission.

JOURNAL OF INFECTION AND CHEMOTHERAPY (2021)

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Article Pathology

Comparison of 12 Molecular Detection Assays for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Yasufumi Matsumura et al.

Summary: Molecular testing is crucial for accurate diagnosis of SARS-CoV-2 infection, with all assays showing 100% specificity and the N2 assays from the US Centers for Disease Control and Prevention and the Japanese National Institute of Infectious Disease being the most sensitive.

JOURNAL OF MOLECULAR DIAGNOSTICS (2021)

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Article Multidisciplinary Sciences

Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study

Masaki Karino et al.

Summary: This study compared the results of genome extraction and detection from nasopharyngeal swab samples using QIAGEN and Eiken extraction kits. The study found that both QIAGEN and Eiken extraction kits showed favorable results with high concordance rates when compared to PCR, indicating they are both reliable methods for SARS-CoV-2 detection. Additionally, the Eiken extraction kit offers the advantage of quicker testing turnaround time, allowing for prompt detection of SARS-CoV-2.

PLOS ONE (2021)

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Article Biochemical Research Methods

Evaluation of three extraction-free SARS-CoV-2 RT-PCR assays: A feasible alternative approach with low technical requirements

Benoit Visseaux et al.

Summary: The study evaluated the performance of different extraction-free SARS-CoV-2 RT-PCR assays, showing good sensitivity, especially for high viral loads (Ct<30), with PrimeScript and Sansure assays performing well.

JOURNAL OF VIROLOGICAL METHODS (2021)

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Article Medical Laboratory Technology

Salivary SARS-CoV-2 antigen rapid detection: A prospective cohort study

Daniela Basso et al.

Summary: Self-collected saliva is a valid alternative to NPS for SARS-CoV-2 detection by molecular testing, and also shows effectiveness in CLEIA antigen testing. CLEIA antigen is highly accurate in distinguishing positive and negative NPS, while saliva POC antigen has limited sensitivity depending on viral loads.

CLINICA CHIMICA ACTA (2021)

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Editorial Material Microbiology

Routine Antigen Testing Is Not a Substitute for Health Care Worker Vaccination against SARS-CoV-2

Mary Elizabeth Sexton et al.

Summary: The utility of rapid antigen testing for SARS-CoV-2 depends on its accuracy and appropriateness in clinical situations to avoid unnecessary isolation due to high false-positive rates.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

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Article Multidisciplinary Sciences

Limit of detection in different matrices of 19 commercially available rapid antigen tests for the detection of SARS-CoV-2

Ana Cubas-Atienzar et al.

Summary: In this study, an independent evaluation was conducted on the limit of detection (LOD) of 19 marketed Ag-RDTs, revealing that most tests performed well with direct culture supernatant but showed decreased sensitivity in Amies media. Additionally, after 7 days of storage at -80 degrees C, some tests exhibited reduced efficiency on stored dilutions of SARS-CoV-2.

SCIENTIFIC REPORTS (2021)

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Editorial Material Medicine, General & Internal