Journal
CHEMICAL COMMUNICATIONS
Volume 51, Issue 32, Pages 6901-6904Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4cc08265f
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Funding
- University of Bath
- EPSRC
- EC (ERC Consolidator Grant O2SENSE)
- Royal Society
- MRC
- STFC
- Science Foundation of China University of Petroleum, Beijing [2462013YJRC029]
- EPSRC Mass Spectrometry Service at Swansea for assistance, STFC
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We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.
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