Journal
TROPICAL MEDICINE AND INFECTIOUS DISEASE
Volume 8, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/tropicalmed8040237
Keywords
West Nile virus infection; Culicidae; Culex; disease vectors
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This study reports the first isolation and genomic sequencing of West Nile virus in arthropods in Brazil, specifically in a species of Culex mosquito. The findings provide evidence of the virus circulating in mosquitoes in Brazil.
West Nile virus is a flavivirus transmitted by mosquitoes, mainly of the genus Culex. In Brazil, serological studies have already indicated the circulation of the virus since 2003, with the first human case detected in 2014. The objective of the present paper is to report the first isolation of WNV in a Culex (Melanoconion) mosquito. Arthropods were collected by protected human attraction and CDC light bait, and taxonomically identified and analyzed by viral isolation, complement fixation and genomic sequencing tests. WNV was isolated from samples of Culex (Melanoconion) mosquitoes, and the sequencing analysis demonstrated that the isolated strain belonged to lineage 1a. The finding of the present study presents the first evidence of the isolation and genome sequencing of WNV in arthropods in Brazil.
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