Structural Identifiability and Observability of Microbial Community Models

Biological communities are populations of various species interacting in a common location. Microbial communities, which are formed by microorganisms, are ubiquitous in nature and are increasingly used in biotechnological and biomedical applications. They are nonlinear systems whose dynamics can be accurately described by models of ordinary differential equations (ODEs). A number of ODE models have been proposed to describe microbial communities. However, the structural identifiability and observability of most of them-that is, the theoretical possibility of inferring their parameters and internal states by observing their output-have not been determined yet. It is important to establish whether a model possesses these properties, because, in their absence, the ability of a model to make reliable predictions may be compromised. Hence, in this paper, we analyse these properties for the main families of microbial community models. We consider several dimensions and measurements; overall, we analyse more than a hundred different configurations. We find that some of them are fully identifiable and observable, but a number of cases are structurally unidentifiable and/or unobservable under typical experimental conditions. Our results help in deciding which modelling frameworks may be used for a given purpose in this emerging area, and which ones should be avoided.

Automated summary

Microbial communities, composed of microorganisms, are widely distributed in nature and increasingly applied in biotechnology and biomedicine. This study analyzes the structural identifiability and observability of various microbial community models and finds that some models are fully identifiable and observable, while others are structurally unidentifiable and/or unobservable under typical experimental conditions. These findings provide guidance for selecting appropriate modeling frameworks in this emerging field and avoiding inappropriate models.