Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 67, Issue -, Pages 231-236Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2016.04.082
Keywords
Fluorescence confocal microscopy; Silica Quantum Dots; Chondrocyte bovine cells; Hyaluronic acid; Labelling; Long term cell staining
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Funding
- sicilian project Piattaforma regionale di ricerca traslazione per la salute [P.O. FESR 2007/2013 - 4.1.2.A - CUP B65E12000570008]
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Quantum Dots (QDs) are promising very bright and stable fluorescent probes for optical studies in the biological field but water solubility and possible metal bio-contamination need to be addressed. In this work, a simple silica-QD hybrid system is prepared and the uptake in bovine chondrocytes living cells without any functionalization of the external protective silica shield is demonstrated. Moreover, long term treated cells vitality (up to 14 days) and the transfer of silica-QDs to the next cell generations are here reported. Confocal fluorescence microscopy was also used to determine the morphology of the so labelled cells and the relative silica-QDs distribution. Finally, we employ silica-QD stained chondrocytes to characterize, as proof of concept, hydrogels obtained from an amphiphilic derivative of hyaluronic acid (HA-EDA-C-18) functionalized with different amounts of the RGD peptide. (C) 2016 Elsevier B.V. All rights reserved.
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