Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 59, Issue -, Pages 185-192Publisher
ELSEVIER
DOI: 10.1016/j.msec.2015.10.013
Keywords
Silk fibroin; Hydrogel; Fibrous
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Funding
- Innovation and Entrepreneurship Program of Qingdao University [201511065004]
- National Natural Science Foundation of Shandong Province [BS2015CL017]
- National Natural Science Foundation of China [81271723]
- National Engineering Laboratory for Modern Silk
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Hydrogels prepared by silk fibroin solution have been studied. However, mimicking the nanofibrous structures of extracellular matrix for fabricating biomaterials remains a challenge. Here, a novel two-step method was applied to prepare fibrous hydrogels using regenerated silk fibroin solution containing nanofibrils in a range of tens to hundreds of nanometers. When the gelation process of silk solution occurred, it showed a top-down type gel within 30 min. After gelation, silk fibroin fibrous hydrogels exhibited nanofiber network morphology with beta-sheet structure. Moreover, the compressive stress and modulus of fibrous hydrogels were 31.9 +/- 2.6 and 2.8 +/- 0.8 kPa, respectively, which was formed using 2.0 wt.% concentration solutions. In addition, fibrous hydrogels supported BMSCs attachment and proliferation over 12 days. This study provides important insight in the in vitro processing of silk fibroin into useful new materials. (C) 2015 Elsevier B.V. All rights reserved.
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