Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 64, Issue -, Pages 34-42Publisher
ELSEVIER
DOI: 10.1016/j.msec.2016.03.059
Keywords
Gold nanoparticle; Dynamic flow; Interstitial fluid; Tannic acid; Cytotoxicity; Nano-cellular interface
Categories
Funding
- Repperger Research Intern Program through the Air Force Chief Scientist's Office
- Summer Faculty Fellowship Program through the Air Force Office of Scientific Research
- Summer Faculty Fellowship Program through the University of Dayton Research Council Seed Grant
- Oak Ridge Institute for Science and Education (ORISE)
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Nanoparticle (NP) effects in a biological system are driven through the formation and structure of the protein corona-NP complex, which is dynamic by nature and dependent upon factors from both the local environment and NP physicochemical parameters. To date, considerable data has been gathered regarding the structure and behavior of the protein corona in blood, plasma, and traditional cell culture medium. However, there exists a knowledge gap pertaining to the protein corona in additional biological fluids and following incubation in a dynamic environment. Using 13 nm gold NPs (AuNPs), functionalized with either polyethylene glycol or tannic acid, we demonstrated that both particle characteristics and the associated protein corona were altered when exposed to artificial physiological fluids and under dynamic flow. Furthermore, the magnitude of observed behavioral shifts were dependent upon AuNP surface chemistry. Lastly, we revealed that exposure to interstitial fluid produced protein corona modifications, reshaping of the nano-cellular interface, modified AuNP dosimetry, and induction of previously unseen cytotoxicity. This study highlights the need to elucidate both NP and protein corona behavior in biologically representative environments in an effort to increase accurate interpretation of data and transfer of this knowledge to efficacy, behavior, and safety of nano-based applications. (C) 2016 Elsevier B.V. All rights reserved.
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