Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 68, Issue -, Pages 52-58Publisher
ELSEVIER
DOI: 10.1016/j.msec.2016.05.107
Keywords
Cardiovascular disease; Myoglobin; Immunosensors; Electrochemical impedance spectroscopy; Screen-printed multiwalled carbon nanotubes electrode
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Funding
- Department of Science and Technology (DST), Government of India [INT/RUS/RFBR/P-153, SERB-DST SB/S2/RJN-087/2014]
- Project of Russian Ministry of Science and Education [14.576.21.0045, RFMEF157614X0045]
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We report the construction and characterization of a novel, level free impedimetric immunosensor for rapid, sensitive and selective detection of myoglobin (Mb). Monoclonal anti-myoglobin (anti-Mb-IgG) antibody was immobilized on screen-printed multiwalled carbon nanotubes electrode for signal amplification without the need of natural enzymes. The fabrication of resulting immunosensor was extensively characterized by using scanning electron microscopy (SEM), fourier transform infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Electrochemical impedance spectroscopy (EIS) technique offered a linear detection range (0.1-90 ng mL(-1)) of myoglobin with sensitivity of 0.74 k Omega ng mL(-1) (correlation coefficient, R-2 = 0.97) and detection limit of 0.08 ng mL(-1) (S/N = 3). The mean percentage recovery of Mb in serum samples using this working biosensor is 97.33%. Furthermore, the proposed strategy can be a promising alternative for detection of Mb related cardiovascular disorders. (C) 2016 Elsevier B.V. All rights reserved.
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