Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 59, Issue -, Pages 731-739Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2015.10.060
Keywords
Magnetosome; Acidithiobacillus ferrooxidans; Response surface methodology; Desirability function; Optimization
Categories
Funding
- National Natural Science Foundation of China [31100006, 41471201]
- Natural Science Foundation of Heilongjiang Province of China [QC2014C023, C201442]
- University Nursing Program for Young Scholars with Creative Talents in Heliongliang Province [UNPYSCT-2015086]
- Program of Science and Technology Innovation Teams Building in Heilongjiang Province [2012TD006]
- China Scholarship Council (CSC) [201308230032]
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Present study aimed to resolve the conflict between cell growth and magnetosome formation of Acidithiobocillus ferrooxidans (A. ferrooxidans) in batch experiments by applying response surface methodology (RSM) integrated a desirability function approach. The effects of several operating parameters on cell growth (OD600) and magnetosome production (C-mag) were evaluated. The maximum overall desirability (D) of 0.923 was achieved at iron concentration of 125.07 mM, shake speed of 12237 rpm and nitrogen concentration of 2.40 g/L Correspondingly, the OD500 and C-mag were 0.522 and 1,196, respectively. The confirmation experiment confirmed that the optimum OD600 and C-mag obtained were in good agreement with the predicted values. The inductively coupled plasma atomic emission spectrometer (ICP-AES) and transmission electron microscopy (TEM) analyses revealed that the production of magnetosomes could be improved via optimization. X-ray diffraction (XRD) showed the magnetosomes are magnetite. Results indicated that RSM with a desirability function was a useful technique to get the maximum OD600 and C-mag simultaneously. (C) 2015 Elsevier B.V. All rights reserved.
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