Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 59, Issue -, Pages 10-18Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2015.09.074
Keywords
Adhesion; Cardiac tissue engineering; Gold nanotube/nanowire; Nanocomposite; Biodegradable polyurethane; Electrical stimulation
Categories
Funding
- Boehringer Ingelheim Fonds
- Deutsche Forschungsgemeinschaft (DFG) [SFB 677, SE 1801/2-1]
- European Research Council (ERC) [336104]
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Following a myocardial infarction (MI), cardiomyocytes are replaced by scar tissue, which decreases ventricular contractile function. Tissue engineering is a promising approach to regenerate such damaged cardiomyocyte tissue. Engineered cardiac patches can be fabricated by seeding a high density of cardiac cells onto a synthetic or natural porous polymer. In this study, nanocomposite scaffolds made of gold nanotubes/nanowires incorporated into biodegradable castor oil-based polyurethane were employed to make micro-porous scaffolds. H9C2 cardiomyocyte cells were cultured on the scaffolds for one day, and electrical stimulation was applied to improve cell communication and interaction in neighboring pores. Cells-on scaffolds were examined by fluorescence microscopy and scanning electron microscopy, revealing that the combination of scaffold design and electrical stimulation significantly increased cell confluency of H9C2 cells on the scaffolds. Furthermore, we showed that the gene expression levels of Nkx2.5, atrial natriuretic peptide (ANF) and natriuretic peptide precursor B (NPPB), which are functional genes of the myocardium, were up-regulated by the incorporation of gold nanotubes/nanowires into the polyurethane scaffolds, in-particular after electrical stimulation. (C) 2015 The Authors. Published by Elsevier B.V.
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