Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 58, Issue -, Pages 97-102Publisher
ELSEVIER
DOI: 10.1016/j.msec.2015.08.013
Keywords
Hemoglobin; Direct electron transfer; Third generation biosensor; Silver nanoparticles; Hydrogen peroxide
Categories
Funding
- FAPESP [12/02877-4]
- CNPq [441428/2014-2]
- CAPES
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A new architecture for a biosensor is proposed using a glassy carbon electrode (GCE) modified with hemoglobin (Hb) and silver nanopartides (AgNPs) encapsulated in poly(amidoamine) dendrimer (PAMAM). The biosensors were characterized using ultraviolet-visible spectroscopy, zeta-potential and cyclic voltammetry to investigate the interactions between Hb, AgNPs and the PAMAM film. The biosensor exhibited a well-defined cathodic peak attributed to reduction of the Fe3+ present in the heme group in Hb, as revealed by cyclic voltammetry in the presence of O-2. An apparent heterogeneous electron transfer rate of 4.1 s(-1) was obtained. The Hb-AgNPs-PAMAM/GCE third generation biosensor was applied in the amperometric determination of hydrogen peroxide over the linear range from 6.0 x 10(-6) to 9.1 x 10(-5) mol L-1 with a detection limit of 4.9 x 10(-6) mol L-1. The proposed method can be extended to immobilize and evaluate the direct electron transfer of other redox enzymes. (C) 2015 Elsevier B.V. All rights reserved.
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