Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 60, Issue -, Pages 45-53Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2015.11.012
Keywords
Surface topography; Osteogenic differentiation; hBMSCs; YAP/TAZ; Hydroxyapatite
Categories
Funding
- National Natural Science Foundation of China [81271974, 81572126]
- Natural Science Foundation of Zhejiang Province [LY15H060005]
- Public Project of Zhejiang Province [201403150]
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Effective and safe induction of osteogenic differentiation is one of the key elements of bone tissue engineering. Surface topography of scaffold materials was recently found to promote osteogenic differentiation. Utilization of this topography may be a safer approach than traditional induction by growth factors or chemicals. The aim of this study is to investigate the enhancement of osteogenic differentiation by surface topography and its mechanism of action. Hydroxyapatite (HA) discs with average roughness (Ra) of surface topography ranging from 0.2 to 1.65 mu m and mean distance between peaks (RSm) ranging from 89.7 to 18.6 mu m were prepared, and human bone-marrow mesenchymal stem cells (hBMSCs) were cultured on these discs. Optimal osteogenic differentiation was observed on discs with surface topography characterized by Ra ranging from 0.77 to 1.09 mu m and RSm ranging from 53.9 to 39.3 mu m. On this surface configuration of HA, hBMSCs showed oriented attachment, F-actin arrangement, and a peak in the expression of Yes-associated protein (YAP) and PDZ binding motif (TAZ) (YAP/TAZ). These results indicated that the surface topography of HA promoted osteogenic differentiation of hBMSCs, possibly by increasing cell attachment and promoting the YAP/TAZ signaling pathway. (C) 2015 Elsevier B.V. All rights reserved.
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