Biological (microfloral) factors of influence on cytogenetic stability during chemical mutagenesis

The article presents the results of a study of chromosomal mutations in residents living in the Aral Sea disaster zone. The present study was designed to evaluate the impact of the combined effect of a chemical mutagen (nickel) and bacterial microflora on the level of chromosomal aberrations (CA) in peripheral blood lymphocytes. This study used classical methods of cell cultivation, methods for determining chromosomal aberrations, a cytomorphological method for assessing epithelial cells, and an atomic absorption method for determining trace elements in the blood. The article shows that with an increase of chemical agent in the blood, the number of cells with features of damage and cells with contamination by microflora increases. Both of these factors cause an increase in the frequency of chromosomal aberrations. The article demonstrates how being exposed to a chemical factor increases chromosomal mutations, and also damages membrane components, which leads to a decrease in the barrier and protective function of the cell, and as a result also affects the level of chromosomal aberrations.

Automated summary

The study examines the chromosomal mutations in residents living in the Aral Sea disaster zone, specifically focusing on the combined impact of a chemical mutagen (nickel) and bacterial microflora on the level of chromosomal aberrations. Various methods, including cell cultivation and determination of chromosomal aberrations, were used to analyze the samples. The results show that increased levels of the chemical agent in the blood lead to a higher frequency of chromosomal aberrations due to the damage caused to the cells and contamination by microflora. The study highlights the link between chemical exposure, membrane damage, and increased chromosomal mutations.