Journal
MARINE POLLUTION BULLETIN
Volume 109, Issue 1, Pages 350-355Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2016.05.054
Keywords
eDNA; Brackish water; Rhithropanopeus harrisii; Biological invasions; Detection
Funding
- Societas pro Fauna et Flora Fennica
- Academy of Finland [266321]
- Estonian Ministry of Education and Research [IUT8-2]
- Academy of Finland (AKA) [266321, 266321] Funding Source: Academy of Finland (AKA)
Ask authors/readers for more resources
The need to detect and monitor introduced marine species has increased with the increasing number of marine invasions. To complement standard detection and monitoring techniques, new approaches using environmental DNA (eDNA) have recently been developed. However, most of the eDNA work has focused on vertebrate species in spatially limited freshwater habitats while benthic invertebrates in coastal environments have received much less attention. Here, we evaluated the suitability of the eDNA approach for detecting benthic, hard-shelled, crustacean mud crab species in a brackish water environment. We demonstrated for the first time that eDNA from an introduced mud crab Rhithropanopeus harrisii can be successfully amplified in aquarium water samples and detected in the brackish water environment. However, the detection rate was rather low. This suggests that in contrast to freshwater vertebrates, it may be more challenging to develop a highly sensitive eDNA method for detecting crustacean species in a marine environment. (C) 2016 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available