Journal
MARINE POLLUTION BULLETIN
Volume 108, Issue 1-2, Pages 53-61Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2016.04.063
Keywords
PLHC-1 cells; Sediments; Cytotoxicity; CYP1A induction; PXR agonists; Biomonitoring
Funding
- Policy-oriented marine Environmental Research in the Southern European Seas (PERSEUS) [287600]
- NILS Science and Sustainability [ABEL-CM-01-2013, ES07]
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The environmental quality of marine sediments collected in the area of influence of the Po and Danube Rivers was assessed by using a battery of bioassays based on the use of PLHC-1 cells, zebrafish-Pxr-transfected COS-7 cells, and sea bass ovarian subcellular fractions. This allowed the determination of multiple endpoints, namely, cytotoxicity, oxidative stress, induction of CYP1A, activation of zebrafish Pxr and inhibition of ovarian aromatase. Organic extracts of sediments influenced by the Danube River and collected near harbors and urban discharges showed significant cytotoxicity, CYP1A induction and inhibition of aromatase activity. An analogous response of CYP1A induction and zfPxr activation was observed, which suggests the existence of common ligands of AhR and PXR in the sediment extracts. The study highlights the usefulness of the selected bioassays to identify those sediments that could pose a risk to aquatic organisms and that require further action in order to improve their environmental quality. (C) 2016 Elsevier Ltd. All rights reserved.
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