Journal
MARINE GENOMICS
Volume 30, Issue -, Pages 55-65Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.margen.2016.10.003
Keywords
Genome editing; CRISPR/Cas9; Model organisms; Non-model organisms; Functional genomics
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Funding
- ANR [ANR-II-INBS-0014]
- Projet Exploratoire Collaborative grant from SATS-Sorbonne Universites/Andre Picard network [ANR-11-IDEX-0004-02]
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More and more genomes are sequenced and a great range of biological questions can be examined at the genomic level in a growing number of organisms. Testing the function of genome features, from gene networks, genome organization, conserved non-coding sequences to microRNAs, and, more generally, experimentally addressing the genotype-phenotype relationship is now possible owing to the clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9 revolution of genome editing. In the present review, we give a brief overview of the CRISPR/Cas9 toolbox and different strategies for genome editing currently available. We list the first examples of applications to marine organisms and also draw from studies in more common laboratory models to suggest both guidelines for design of genome editing experiments as well as discuss challenges specific to marine organisms. In addition, we discuss future perspectives, including applications of CRISPR/Cas9 to base editing and targeted reprogramming of gene transcription. (C) 2016 Elsevier B.V. All rights reserved.
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