In silico microRNA network data in zebrafish after antineoplastic ifosfamide exposure

Ifosfamide is a cancer-fighting chemotherapeutic that has been detected in aquatic ecosystems. Zebrafish larvae were exposed to either 0, 1 or 100 mu g/L ifosfamide in the water for 7 days, and fish were subjected to total RNA extraction and RNA-seq analysis with the Illumina NovoSeq 60 0 0 in-strument. Raw sequence data were processed through fastp and clean reads obtained by removing adapter and poly-N sequences, as well as low quality reads. Differential gene expression was performed using the abundance of tran-scripts that mapped to the zebrafish genome. To uncover putative targets regulated by microRNAs, Pathway Studio 12.0 was used to conduct a subnetwork enrichment anal-ysis. Expression data were used to predict which microR-NAs were important for the response to ifosfamide expo-sure. There were 21 common microRNAs identified in both the IFOS1 and IFOS100 datasets. These were MIR150, MIR6515, MIR657, MIR216A, m_Mir741, MIRLET7E, miR-let-7, MIR2392, r_Mir3551, MIR181B1, MIR33A, MIR502, MIR193B, MIR146A, MIR431, MIR647, m_Mir1192, MIR297, MIR328, and MIR4717. Data can be re-used to advance adverse outcome pathways in regulatory toxicology and to refine biomarker discovery for antineoplastics in aquatic environments.(c) 2023 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )

Automated summary

Zebrafish larvae were exposed to different concentrations of Ifosfamide, and RNA-seq analysis revealed 21 microRNAs that were associated with Ifosfamide exposure. This study provides valuable insights for the advancement of regulatory toxicology and biomarker discovery in aquatic environments.