Journal
MACROMOLECULAR MATERIALS AND ENGINEERING
Volume 301, Issue 7, Pages 801-804Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mame.201500315
Keywords
autodeposition; enzymes; melanin; nanoparticles
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The previously introduced process for enzyme-mediated in situ synthesis and deposition of eumelanin is investigated with covalent immobilization of the tyrosinase. It results in a monolayer structure of non-coalesced melanin particles, with a film thickness of 5-8 nm. The reaction is self-terminating due to overlay of the enzymes with particles. The melanin particles are rodlike with lengths down to 6 nm. Isolated melanin structures of such small size have not been observed before and might be a kind of protoparticle in the supramolecular buildup of melanin oligomers. Utilization of melanin particles with such small size can enable nanotechnological applications in the areas of bioelectronics and biosensors.
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