Journal
MACROMOLECULAR BIOSCIENCE
Volume 17, Issue 4, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.201600349
Keywords
adhesion; Alaska pollock-derived gelatin; hydrophobically modified gelatinl lung; surgical sealant
Funding
- Japan Society for the Promotion of Science KAKENHI [16H04524]
- Grants-in-Aid for Scientific Research [16H04524] Funding Source: KAKEN
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Pulmonary air leaks are medical complications of thoracic surgery for which fibrin sealant is the main treatment. In this study, innovative sealants based on hydrophobically modified Alaska pollock- derived gelatin (hm-ApGltn) and a poly(ethylene) glycol-based 4-armed cross-linker (4S-PEG) have been developed and their burst strengths have been evaluated using fresh rat lung. The developed sealants show higher lung burst strength compared with the nonmodified original ApGltn (Org-ApGltn)-based sealant and a commercial fibrin sealant. The maximum burst strength of the hm-ApGltn-based sealant is 1.6-fold higher than the Org-ApGltn-based sealant (n = 5, p < 0.05), and 2.1-fold higher than the commercial fibrin sealant (n = 5, p < 0.05). Cell culture experiments show that modification of ApGltn with cholesteryl or stearoyl groups effectively enhances anchoring to the cell surface. In addition, binding constants between hm-ApGltn and extracellular matrix proteins such as fibronectin and fibrillin are increased. Therefore, the new hm-ApGltn/4S-PEG-based sealant has the potential for applications in thoracic surgery.
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