High-level and reusable preparation of sulforaphane by yeast cells expressing myrosinase

Myrosinase is a key tool for the fast and efficient preparation of sulforaphane which is one of the prominent natural ingredients found in brassicaceous vegetables. Here, the glucoraphanin-hydrolyzing activity of a Yarrowia lipolytica 20-8 harboring myrosinase reached 73.28 U/g dry cell weight, indicating that it had a potential application in sulforaphane preparation from glucoraphanin. An efficient and reusable process for sulforaphane preparation via myrosinase produced by Y. lipolytica 20-8 was constructed. In detail, as high as 10.32 mg sulforaphane could be produced from 1 g broccoli seed under the reaction of 40 U yeast whole-cell catalyst within 15 min with the conversion efficiency of 99.86%. Moreover, when the yeast whole-cell catalyst was reused 7 and 10 times, as high as 92.53% and 87.56% of sulforaphene yield of the initial level could be retained, respectively. Therefore, this yeast whole-cell is a potent biocatalyst for the efficient and reusable preparation of sulforaphane.

Automated summary

A yeast strain called Yarrowia lipolytica 20-8 with high glucoraphanin-hydrolyzing activity was found to be effective in the preparation of sulforaphane. The reaction efficiency reached 99.86% with a production of 10.32 mg sulforaphane from 1 g broccoli seed in just 15 minutes using a yeast whole-cell catalyst. The catalyst could also be reused multiple times with a retention rate of 87.56-92.53% of the initial sulforaphene yield.