Essential Oil Yield, Composition, and Antioxidant Activity in Two Umbel Maturity Stages of Wild Carrot (Daucus carota L. ssp. carota) from Montenegro

The purpose of this study was to determine essential oil yield, composition, and antioxidant activity during two different maturation stages of umbels with seeds(I stage: premature-waxy; and II stage: mature-fully ripening) of wild carrot (Daucus carrota var. carota) from the Montenegrin coast. A higher yield of carrot essential oil (CEO) was determined in mature, fully ripening umbels (1.96 mL/100 g p.m) than in premature umbels at the waxy stage (mL/100 g p.m). Thirty-three components were identified in premature umbels, with beta-bisabolene (32.3%), 11-alpha-(H)-himachal-4-en-1-beta-ol (27.9%), elemicin (10.1%), and alpha-longipipene (7.7%) being the main components. They were followed by alpha-pinene (3.7%), (E)-asarone (3.4%), (E)-anethole (3.2%), and beta-himachalene (2.0%). Thirty-two components were identified in CEO from mature umbels, with beta-bisabolene (41.0%), 11-alpha-(H)-himachal-4-en-1-beta-ol(21.1%), elemicin (14.8%), and alpha-longipipene (5.7%) being the most abundant. These components were followed by (E)-asarone (3.9%), cis-alpha-bisabolene (2.4%), and beta-himachalene (2.0%). The CEO isolated from mature umbelsshowed better antioxidant activity (EC50 value of 31.80 mg/mL) in comparison to the CEO isolated from premature umbels (EC50 value of 49.18 mg/mL) during the incubation time of 60 min. The degree of DPPH radical neutralization increased as the incubation time increased from 20 to 60 min. Therefore, our findings recommend that wild carrot could be harvested in the fully ripening stage when the umbel improves CEO yield and antioxidant activity, without the risk of seed shedding from the umbel and seed losses.

Automated summary

The purpose of this study was to determine the yield, composition, and antioxidant activity of essential oil from umbels with seeds of wild carrot at different maturation stages. The results showed that mature umbels had a higher yield of essential oil compared to premature umbels. The main components identified in both premature and mature umbels were beta-bisabolene, 11-alpha-(H)-himachal-4-en-1-beta-ol, elemicin, and alpha-longipipene. The antioxidant activity of essential oil from mature umbels was also found to be higher than that from premature umbels during a 60-minute incubation.