Microbiome Characterization after Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge

A demonstrator plant of a recently patented process for improved sludge degradation has been implemented on a municipal scale. In a 1500 m(3) sewage sludge digester, an intermediary stage with aerobic sewage sludge reactivation was implemented. This oxic activation increased the biogas yield by up to 55% with a 25% reduction of the remaining fermentation residue volume. Furthermore, this process allowed an NH4-N removal of over 90%. Additionally, 16S rRNA gene amplicon high-throughput sequencing of the reactivated digestate showed a reduced number of methane-forming archaea compared to the main digester. Multiple ammonium-oxidizing bacteria were detected. This includes multiple genera belonging to the family Chitinophagaceae (the highest values reached 18.8% of the DNA sequences) as well as a small amount of the genus Candidatus nitrosoglobus (<0.3%). In summary, the process described here provides an economically viable method to eliminate nitrogen from sewage sludge while achieving higher biogas yields and fewer potential pathogens in the residuals.

Automated summary

A demonstrator plant for an improved sludge degradation process has been implemented on a municipal scale. By introducing an aerobic sewage sludge reactivation stage, the biogas yield increased by 55% and the remaining fermentation residue volume reduced by 25%. NH4-N removal of over 90% was achieved, and the reactivated digestate showed a reduced number of methane-forming archaea and presence of ammonium-oxidizing bacteria.