Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 73, Issue -, Pages 290-295Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.lwt.2016.06.026
Keywords
Phospholipase A(2); Magnetic immobilization; Oil degumming; Free fatty acids
Categories
Funding
- National Natural Science Foundation of China (NSFC) [31571880]
- Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, China
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In this study, magnetic immobilized phospholipase A(2) (PLA(2)) was used to degum soybean oil such as magnetic particles crosslinked with sodium alginate PLA(2) (PLA(2)-MPCSA), Magnetic chitosan micropartides containing PLA(2) (PLA(2)-MCM), Fe3O4/P(GMA-EDGM-St) PLA(2), and Fe3O4/SiOx-g-P(GMA) PLA(2), successfully improving the recycling aspect of the degumming process. The highest enzyme loading and enzymatic activity (122.60 mg/g and 1289 U/g, respectively) were obtained using the magnetic immobilized PLA(2)-Fe3O4/SiOx-g-P(GMA) system. Compared to free PLA(2), magnetic immobilized PLA(2) had a broader pH-activity profile (pH 4.0-5.5) and activity remained stable between 50 and 70 degrees C. Enzymatic degumming with PLA(2)-Fe3O4/SiOx-g-P(GMA) was carried out over 5.0 h with an enzyme dosage of 0.24 g/kg, a pH of 5.0, and a temperature of 55 degrees C, resulting in residual phosphorus and free fatty acid contents of 9.8 mg/kg and 0.84 g/100 g, respectively. Moreover, magnetic immobilized PLA(2) retained more than 80% of its initial activity even after 5 cycles of soybean oil degumming. (C) 2016 Elsevier Ltd. All rights reserved.
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