GABA-enriched water dropwort produced by co-fermentation with Leuconostoc mesenteroides SM and Lactobacillus plantarum K154

Water dropwort (Oenanthe javanica DC) was co-fermented with Leuconostoc mesenteroides SM and Lactobacillus plantarum K154 to produce a novel functional food ingredient enriched with dextran and gamma-aminobutyric acid (GABA). Dextran production was maximised with a water: dropwort ratio of 80: 20 (w/w) and 20% sucrose, reaching a consistency index of 6 Pa.s(n) after fermenting for 3 days at 25 degrees C. To achieve a successful second fermentation, L. plantarum 1054 was cultured in the presence of 3% monosodium glutamate (MSG) and 0.25% yeast extract for 7 days, resulting in a pH of 4.14,1.6% acidity, and a viable cell count of 8.04 log CFU/mL. Also, a yield of 10 mg/mL of GABA was achieved by the efficient bioconversion of the MSG substrate. In conclusion, fermented water dropwort as a functional food ingredient containing dextran, GABA, and probiotics was efficiently produced by consecutive co-fermentation using Leu. mesenteroides SM and L plantarum K154. (C) 2016 Published by Elsevier Ltd.