Journal
FOOD SCIENCE AND HUMAN WELLNESS
Volume 12, Issue 2, Pages 407-415Publisher
TSINGHUA UNIV PRESS
DOI: 10.1016/j.fshw.2022.07.042
Keywords
Rosa Chinensis Flos; Polysaccharide isolation; Antithrombotic activity; Intestinal flora
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This study reports the isolation of a polysaccharide (RCJ2-Ib) from Rosa Chinensis Flos, which exhibits significant anticoagulant activity and the ability to maintain intestinal environment.
This is the first report on a polysaccharide (RCJ2-Ib) isolated from Rosa Chinensis Flos. RCJ2-Ib was obtained through the extraction with water, precipitation with ethanol, separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 column. GC, FT-IR and NMR analyses revealed that RCJ2-Ib (3.3 kDa) was a 1,4-linked polymannuronic acid containing substantial beta-D- anomers units. The anticoagulant effect of RCJ2-Ib evaluated by using rabbit ear venous blood and an acute blood stasis rat model showed that RCJ2-Ib had obvious anticoagulant activity in regulating endogenous and exogenous coagulation pathways and reducing serum thromboxane B2 and endothelin-1. In addition, RCJ2-Ib could also increase the number of Lactobacillus and Escherichia coli. As a result, RCJ2-Ib has the potential to inhibit thrombosis and maintain the intestinal environment. (C) 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
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