Electrochemical Mercury Biosensor Based on Electrocatalytic Properties of Prussian Blue and Inhibition of Catalase

This paper presents a detailed study of a novel type of electrochemical mercury ion (Hg2+) biosensor developed by combining Prussian blue (PB) and catalase (Cat). The simultaneous PB-catalyzed reduction of hydrogen peroxide and the inhibition of catalase by Hg2+ ions were used as the working principle of the biosensor. The biosensor described in this research was capable of detecting Hg2+ ions at relatively low potentials (+0.2 V vs. Ag|AgCl, KClsat) using chronoamperometry and a fast Fourier transform electrochemical impedance spectroscopy (FFT-EIS). Linear ranges of 0.07 mM-3 mM and 0.13 mM-0.80 mM of Hg2+ ions were obtained using amperometric and impedimetric techniques, respectively. In the course of this work, an amperometric study of the Hg2+ ion biosensor was also carried out on a real sample (tap water containing Hg2+ ions).

Automated summary

This paper presents a detailed study on a novel electrochemical biosensor for mercury ions (Hg2+), which combines Prussian blue (PB) and catalase (Cat). The biosensor utilizes the simultaneous reactions of PB-catalyzed reduction of hydrogen peroxide and the inhibition of catalase by Hg2+ ions. It can detect Hg2+ ions at relatively low potentials using chronoamperometry and fast Fourier transform electrochemical impedance spectroscopy (FFT-EIS). The linear ranges for Hg2+ ion detection were determined to be 0.07 mM-3 mM and 0.13 mM-0.80 mM using amperometric and impedimetric techniques, respectively. Additionally, an amperometric study was conducted on a real sample of tap water containing Hg2+ ions.