☆ 4.7 Article

On-Chip Nucleic Acid Purification Followed by ddPCR for SARS-CoV-2 Detection

BIOSENSORS-BASEL (2023)

Related references

Note: Only part of the references are listed.

Article Biophysics

Continuous polymerase chain reaction microfluidics integrated with a gold-capped nanoslit sensing chip for Epstein-Barr virus detection

Han-Yun Hsieh et al.

Summary: This study successfully detected the DNA sequence of LMP1 using a combination of microfluidic PCR and gold nanoslit-based SPR sensor, achieving rapid and accurate analytical procedures, as well as enhancing the detection efficiency of DNA extracted from EBV-positive cells.

BIOSENSORS & BIOELECTRONICS (2022)

Add to Collection

Article Chemistry, Analytical

Easy-to-Operate Co-flow Step Emulsification Device for Droplet Digital Polymerase Chain Reaction

Chunyang Wei et al.

Summary: In this study, a microfluidic chip based on co-flow step emulsification was proposed for droplet digital PCR (ddPCR), which combines droplet generation, PCR amplification, and fluorescence detection on a single device. The chip offers low cost, easy operation, excellent quantitative capability, and small size, making it a promising tool for DNA molecule quantification.

ANALYTICAL CHEMISTRY (2022)

Add to Collection

Article Biophysics

Advantages of optical fibers for facile and enhanced detection in droplet microfluidics

Sundar Hengoju et al.

Summary: Droplet microfluidics using optical fiber as a light guiding medium has revolutionized analytical possibilities by providing a simpler, more flexible, lower-cost, and sensitive setup for the detection of various parameters. This technology has great potential in biochemistry and biotechnology research.

BIOSENSORS & BIOELECTRONICS (2022)

Add to Collection

Review Chemistry, Analytical

Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2

Weidu Song et al.

Summary: This paper introduces the crucial role of nucleic acid testing (NAT) in containing the spread of SARS-CoV-2 and provides a comprehensive review and comparison of various detection technologies, including traditional techniques and microfluidic-based technologies, discussing their characteristics and development prospects.

MICROMACHINES (2022)

Add to Collection

Article Nanoscience & Nanotechnology

A microfluidic system for rapid nucleic acid analysis based on real-time convective PCR at point-of-care testing

Donglin Xu et al.

Summary: A microfluidic system based on real-time convective PCR has been developed for rapid and automated nucleic acid extraction, amplification, and in-situ detection, demonstrating improved detection efficiency and accuracy.

MICROFLUIDICS AND NANOFLUIDICS (2022)

Add to Collection

Article Medical Laboratory Technology

Rapid and Extraction-Free Detection of SARS-CoV-2 from Saliva by Colorimetric Reverse-Transcription Loop-Mediated Isothermal Amplification

Matthew A. Lalli et al.

Summary: The study developed a rapid colorimetric assay for SARS-CoV-2 detection using saliva samples without RNA extraction, achieving high accuracy and sensitivity.

CLINICAL CHEMISTRY (2021)

Add to Collection

Article Microbiology

Clinical Performance of the Point-of-Care cobas Liat for Detection of SARS-CoV-2 in 20 Minutes: a Multicenter Study

Glen Hansen et al.

Summary: The study compared the clinical performance of a 20-minute point-of-care RT-PCR test for SARS-CoV-2 with the widely used laboratory test, showing a high level of agreement between the two methods. Rapid RT-PCR testing at point of care enables timely infection control and individual care decisions for COVID-19.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

Add to Collection

Article Biotechnology & Applied Microbiology

Comparison of Digital PCR and Quantitative PCR with Various SARS-CoV-2 Primer-Probe Sets

Changwoo Park et al.

Summary: The study found that ddPCR is more sensitive than RT-qPCR in detecting viral RNA, especially for determining the copy number of reference materials.

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY (2021)

Add to Collection

Article Biochemical Research Methods

Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction

Carolina Beltran-Pavez et al.

Summary: This study evaluated the sensitivity and performance of different RT-qPCR kits for detecting SARS-CoV-2, explored the feasibility of directly detecting viral RNA in NSS, and proposed a detection protocol that omits the RNA extraction step for faster and more efficient COVID-19 diagnosis.

JOURNAL OF VIROLOGICAL METHODS (2021)

Add to Collection

Article Multidisciplinary Sciences

Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification

Harish N. Vasudevan et al.

Summary: The study demonstrates the accurate and consistent measurement of SARS-CoV-2 viral load using digital droplet PCR (ddPCR) directly from crude lysate without nucleic acid purification. ddPCR performs well in nasopharyngeal swab specimens with low input viral copy numbers, showing higher sensitivity and robustness compared to qPCR when using crude lysate.

SCIENTIFIC REPORTS (2021)

Add to Collection

Article Biochemical Research Methods

Isolation of target DNA using synergistic magnetic bead transport and electrokinetic flow

Lindsay Schneider et al.

Summary: This research introduces a novel microfluidic method (MSEP) to simplify and streamline the process of DNA library preparation for sequencing, effectively reducing hands-on time and pipetting while maintaining high efficiency in eliminating adapter-dimers.

BIOMICROFLUIDICS (2021)

Add to Collection

Review Medicine, General & Internal

Diagnostic, Prognostic, and Therapeutic Value of Droplet Digital PCR (ddPCR) in COVID-19 Patients: A Systematic Review

Angela Ishak et al.

Summary: This study systematically reviewed the diagnostic performance of droplet digital PCR (ddPCR) in COVID-19 patients, showing that ddPCR is more accurate in detecting and quantifying SARS-CoV-2 levels compared to RT-qPCR, especially in patients with low viral loads. Additionally, ddPCR was found to be highly effective in predicting patient response to therapy and monitoring disease progression.

JOURNAL OF CLINICAL MEDICINE (2021)

Add to Collection

Article Obstetrics & Gynecology

Liquid biopsy with droplet digital PCR targeted to specific mutations in plasma cell-free tumor DNA can detect ovarian cancer recurrence earlier than CA125

Takamichi Minato et al.

Summary: By detecting circulating tumor DNA using ddPCR technology, early detection of tumor recurrence in OC patients during follow-up is possible. Tumor-specific mutations identified through exome sequencing can be used to select ddPCR probes, improving detection accuracy. ddPCR showed positive results earlier in the detection of OC recurrence compared to CA125.

GYNECOLOGIC ONCOLOGY REPORTS (2021)

Add to Collection

Article Chemistry, Analytical

Point-of-Care PCR Assays for COVID-19 Detection

Niharika Gupta et al.

Summary: Molecular diagnostics, including RT-PCR and qRT-PCR, have been vital in the global response to the COVID-19 pandemic. In addition to these gold standards, faster antigen tests and other point-of-care devices have also played a crucial role in containing the spread of the virus. The development of miniaturized, high-throughput PCR systems, digital PCR, electrochemical PCR, and CRISPR/Cas technology for POC diagnostics are areas of increasing interest and research focus. Commercial RT-PCR POC systems approved for COVID-19 detection by various agencies have also been discussed.

BIOSENSORS-BASEL (2021)

Add to Collection

Article Chemistry, Analytical

A lab-on-a-chip platform for integrated extraction and detection of SARS-CoV-2 RNA in resource-limited settings

Pablo Rodriguez-Mateos et al.

Summary: Efforts are needed to develop rapid and accurate diagnostic tools for extensive testing of COVID-19. Current gold standard nucleic acid tests require trained personnel and specialist instrumentation, which hampers turnaround time and test accessibility, especially in low-resource settings.

ANALYTICA CHIMICA ACTA (2021)

Add to Collection

Article Biophysics

Ultrafast multiplexed detection of SARS-CoV-2 RNA using a rapid droplet digital PCR system

Hao Yin et al.

Summary: The rapid digital PCR system combines droplet digital and rapid PCR techniques for efficient, accurate, and quantitative detection of SARS-CoV-2 RNA. Benchmark tests with serial diluted reference samples demonstrate the high accuracy of the system, while qPCR may yield significant variations in Ct values leading to false-negative results.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Article Multidisciplinary Sciences

Oil immersed lossless total analysis system for integrated RNA extraction and detection of SARS-CoV-2

Duane S. Juang et al.

Summary: The authors report the development of OIL-TAS, a system that integrates RNA extraction and detection into a single device, which shows promising performance in clinical sample testing with the potential to offer a faster, cheaper, and easier alternative for infectious disease testing.

NATURE COMMUNICATIONS (2021)

Add to Collection

Article Chemistry, Analytical

Smartphone-Based Droplet Digital LAMP Device with Rapid Nucleic Acid Isolation for Highly Sensitive Point-of-Care Detection

Fei Hu et al.

ANALYTICAL CHEMISTRY (2020)

Add to Collection

Article Chemistry, Analytical

On-chip RT-LAMP and colorimetric detection of the prostate cancer 3 biomarker with an integrated thermal and imaging box

Lin-Xiang Wang et al.

TALANTA (2020)

Add to Collection

Article Immunology

ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimens

Tao Suo et al.

EMERGING MICROBES & INFECTIONS (2020)

Add to Collection

Article Biochemical Research Methods

Spontaneous droplet generation via surface wetting

Fengyi Liu et al.

LAB ON A CHIP (2020)

Add to Collection

Article Biochemical Research Methods

A sample-in-multiplex-digital-answer-out chip for fast detection of pathogens

Juxin Yin et al.

LAB ON A CHIP (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Super-Resolution Lensless Imaging of Cells Using Brownian Motion

Yuan Fang et al.

APPLIED SCIENCES-BASEL (2019)

Add to Collection

Article Biochemistry & Molecular Biology

A comparison of droplet digital polymerase chain reaction (PCR), quantitative PCR and metabarcoding for species-specific detection in environmental DNA

Susanna A. Wood et al.

MOLECULAR ECOLOGY RESOURCES (2019)

Add to Collection

Review Chemistry, Analytical

Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology

Joel Sanchez Barea et al.

MICROMACHINES (2019)

Add to Collection

Article Chemistry, Analytical

A fast nucleic acid extraction system for point-of-care and integration of digital PCR

Juxin Yin et al.

ANALYST (2019)

Add to Collection

Article Chemistry, Analytical

Highly efficient DNA extraction and purification from olive oil on a washable and reusable miniaturized device

Joana Carvalho et al.

ANALYTICA CHIMICA ACTA (2018)

Add to Collection

Article Engineering, Electrical & Electronic

Genomic DNA extraction from whole blood using a digital microfluidic (DMF) platform with magnetic beads

Ping-Yi Hung et al.

MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS (2017)

Add to Collection

Article Multidisciplinary Sciences

Integrated DNA and RNA extraction using magnetic beads from viral pathogens causing acute respiratory infections

Hui He et al.

SCIENTIFIC REPORTS (2017)

Add to Collection

Article Microbiology

Comparative Study of GeneXpert MTB/RIF Assay and Multiplex PCR Assay for Direct Detection of Mycobacterium tuberculosis in Suspected Pulmonary Tuberculosis Patients

Anil Kumar Sah et al.

CURRENT MICROBIOLOGY (2017)

Add to Collection

Article Chemistry, Analytical

A Rapid and Automated Device for Purifying Nucleic Acids

Masayuki Uehara et al.

ANALYTICAL SCIENCES (2016)

Add to Collection

Article Chemistry, Analytical

Selective Nucleic Acid Removal via Exclusion (SNARE): Capturing mRNA and DNA from a Single Sample

Lindsay Strotman et al.

ANALYTICAL CHEMISTRY (2013)

Add to Collection

Review Biochemical Research Methods

Surfactants in droplet-based microfluidics

Jean-Christophe Baret

LAB ON A CHIP (2012)

Add to Collection

Article Biochemical Research Methods

One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST)

Scott M. Berry et al.

LAB ON A CHIP (2011)

Add to Collection

Article Pathology

Immiscible Phase Nucleic Acid Purification Eliminates PCR Inhibitors with a Single Pass of Paramagnetic Particles through a Hydrophobic Liquid

Kunal Sur et al.

JOURNAL OF MOLECULAR DIAGNOSTICS (2010)

Add to Collection

Review Infectious Diseases

False-positive results and contamination in nucleic acid amplification assays: Suggestions for a prevent and destroy strategy

A Borst et al.

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES (2004)

Add to Collection

© Peeref 2019-2024. All rights reserved.