Journal
BIOSENSORS-BASEL
Volume 13, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/bios13030381
Keywords
rheumatoid arthritis; immunosensing; electrochemical platform; magnetic microbeads; multiplex; autoantibodies
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In this study, an electrochemical immunoplatform was developed and applied for the simultaneous detection of four biomarkers for rheumatoid arthritis. Magnetic beads were used to immobilize specific antigens, and sandwich-type immunoassays were employed for the detection of the biomarkers. The results were validated against ELISA tests and certified values.
Rheumatoid arthritis (RA) is a systemic chronic autoimmune inflammatory disease that is characterized by the destruction of bone and production of autoantibodies such as rheumatoid factor (RF) and anticitrullinated protein antibodies (ACPAs). The high prevalence of this disease and the need of affordable tools for its early detection led us to prepare the first electrochemical immunoplatform for the simultaneous determination of four RA biomarkers, the autoantibodies: RF, anti-peptidyl-arginine deiminase enzyme (anti-PAD4), anti-cyclic citrullinated peptide (anti-CCP), and anti-citrullinated vimentin (anti-MCV). Functionalized magnetic beads (MBs) were used to immobilize the specific antigens, and sandwich-type immunoassays were implemented for the amperometric detection of the four autoantibodies, using the horseradish peroxidase (HRP)/H2O2/hydroquinone (HQ) system. The immunoplatform was applied to the determination of the biomarkers in human serum of twenty-two patients diagnosed with RA and four healthy individuals, and the results were validated against ELISA tests and the certified values.
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