Response of Coccomyxa cimbrica sp.nov. to Increasing Doses of Cu(II) as a Function of Time: Comparison between Exposure in a Microfluidic Device or with Standard Protocols

In this study, we explore how the in vitro conditions chosen to cultivate and observe the long-term (up to 72 h) toxic effect of Cu(II) on the freshwater microalga Coccomyxa cimbrica sp.nov. can affect the dose response in time. We test three different cultivation protocols: (i) under static conditions in sealed glass cells, (ii) in a microfluidic device, where the sample is constantly circulated with a peristaltic pump, and (iii) under continuous agitation in plastic falcons on an orbital shaker. The advantage and novelty of this study resides in the fact that each condition can mimic different environmental conditions that alga cells can find in nature. The effect of increasing dose of Cu(II) as a function of time (24, 48, and 72 h) is monitored following chlorophyll a fluorescence intensity from single cells. Fluorescence lifetime imaging experiments are also explored to gain information on the changes induced by Cu(II) in the photosynthetic cycle of this microalga.

Automated summary

This study investigates the impact of different in vitro conditions on the dose response in time of the long-term toxic effect of Cu(II) on Coccomyxa cimbrica sp.nov., a freshwater microalga. Three different cultivation protocols were tested to mimic various environmental conditions. The dose response of Cu(II) over time was monitored through chlorophyll a fluorescence intensity and fluorescence lifetime imaging, providing insights into the changes induced by Cu(II) in the microalga's photosynthetic cycle.