Reduced Survival and Disruption of Female Reproductive Output in Two Copepod Species (Acartia clausi and A. tonsa) Exposed to the Model Endocrine Disruptor 17α-Ethinylestradiol

Estuaries are heavily impacted by pollutants from different sources such as urban sewage, industrial waste and agricultural runoff. Endocrine-disrupting chemicals (EDCs) are very concerning pollutants to estuarine wildlife, but little is known about their impact on microscopic biota such as zooplankton. The aim of this work was to investigate the effects of a model EDC, the 17 alpha-ethinylestradiol (EE2), on two copepod species inhabiting the Basque coast (Southeastern Bay of Biscay) estuaries: Acartia clausi (autochthonous neritic species) and Acartia tonsa (non-indigenous brackish species). Female copepods were collected at population maximum time (spring for A. clausi and summer for A. tonsa) and exposed individually to 5 ng/L (low), 5 mu g/L (medium) and 500 mu g/L EE2 (high) doses, from environmental concentrations found in sewage effluents to toxicological concentrations. After 24 h exposure, the survival rate of experimental individuals was checked and the lethal concentration LC50 was calculated. The number of egg-producing females and the amount of egg laying and egg hatching were recorded. The integrated biomarker index (IBR) was calculated to integrate the overall effects of EE2 exposure. Both species had reduced survival rates at 500 mu g/L, and the LC50 was lower in A. tonsa (158 mu g/L) compared to A. clausi (398 mu g/L). The number of eggs laid was significantly reduced in A. clausi at EE2 medium and high doses, while a reduction in the number of eggs in A. tonsa was observed only at the high dose. However, no significant differences were detected in the egg hatching success of exposed A. clausi and A. tonsa. IBR index showed that EE2 had the most detrimental effects on A. tonsa and A. clausi females at the 500 mu g/L dose. In conclusion, after 24 h of exposure, EE2 reduced female copepod survival and disrupted reproductive output, but only at high non-environmentally relevant concentrations.

Automated summary

This study investigated the effects of 17 alpha-ethinylestradiol (EE2) on two copepod species in estuaries. The results showed that EE2 reduced the survival rate and reproductive output of copepods in high concentrations.