Structural, morphological and optical properties at various concentration of Ag doped SiO2-NPs via sol gel method for antibacterial and anticancer activities

Silver doped silicon dioxide (Ag@SiO2) NPs has been acquiring admirable physicochemical and biological properties. Present study enclosed the information about the synthesis of Ag doped (0.0, 0.1, 0.3 and 0.5 g) SiO2NPs via sol-gel method. XRD analysis revealed that the phase change from amorphous to face centered cubic in Ag@SiO2NPs and crystallite size was calculated from 35 to 39 nm by scherrer equation. The irregular and nonuniform surface morphology of Ag@SiO2NPs was observed via SEM micrograph. Presence of oxygen, silicon and silver in Ag doped SiO2 NPs were calculated via EDX. After that the absorbance spectrum of Ag@SiO2NPs was exhibited maxima at 238 nm lies in ultraviolet region was detected by UV-VIS. Moreover, the FTIR analysis depicted different vibrational modes showing asymmetric Si-O-Si, OH and Si-H-O groups attached on surface of NPs. In-vitro bio assay, antibacterial and anticancer activities were judged against gram negative bacteria E.coli and liver HepG2 cancer cell line. Our results revealed maximum inhibition zone 12 mm and most decrease in cell viability (P = 0.025) 44.24% at 40 mg/mL of Ag@SiO2NPs. Overall result shows that by increasing the amount of Ag@SiO2 NPs was boost up the antibacterial and anticancer activities.

Automated summary

This study synthesized silver-doped silicon dioxide nanoparticles (Ag@SiO2NPs) using the sol-gel method and investigated their physicochemical and biological properties. XRD analysis revealed a phase change from amorphous to face-centered cubic in Ag@SiO2NPs with a crystallite size of 35 to 39 nm. SEM images showed an irregular and nonuniform surface morphology of Ag@SiO2NPs. EDX analysis confirmed the presence of oxygen, silicon, and silver in Ag-doped SiO2NPs. UV-VIS spectroscopy detected an absorbance maximum at 238 nm in the UV region. FTIR analysis revealed different vibrational modes indicating the presence of Si-O-Si, OH, and Si-H-O groups on the surface of NPs. Antibacterial and anticancer activities were evaluated against E.coli and HepG2 cancer cell line, showing maximum inhibition zone of 12 mm and a significant decrease in cell viability (P = 0.025) of 44.24% at 40 mg/mL of Ag@SiO2NPs. Overall, the results demonstrate that increasing the amount of Ag@SiO2NPs enhances their antibacterial and anticancer activities.