Characterization of Spontaneous Melanization by Fluorescence Spectroscopy: A Basis for Analytical Application to Biological Substrates

Simple Summary Melanin is a pigment naturally present in various living beings, from animals to plants and fungi. Melanin can be involved in many processes, such as innate immunity and antioxidant protection, including photoprotection and the related abilities to confer color to many biological structures and to interact with light. Light interaction, in particular, is largely exploited to investigate melanin chemical components and precursors for various purposes, from the in situ grading of the pigment for diagnosis in biomedicine to the management of light energy for the development of innovative applications in bioengineering. In this study, the ability of melanin and its precursors to respond to light irradiation have been characterized in terms of changes in the emission light signals, namely fluorescence. Besides the confirmation of the fluorescence emission signature of melanin and of its precursor L-tyrosine, new insights have been provided on intermediates during melanin production and by different conditions of measurement. The subsequent characterization of aqueous extracts from black cat hairs and mosquito eggs proved the value of these experimental procedures for application in the investigation of melanization processes in biological substrates. Melanin is present in various biological substrates where it may participate in several processes, from innate immunity to the still-unsolved opposite roles in antioxidant protection, including photoprotection and the related ability to interact with light. Melanin-light interaction has also been an important source of inspiration for the development of innovative bioengineering applications. These are based on melanin's light-energy-absorption ability of its chemically and structurally complex components and precursors, and on the improvement in analytical and diagnostic procedures in biomedicine. In this regard, here, we characterized the fluorescence spectral properties of melanin and of its precursor L-tyrosine in an aqueous solution during spontaneous melanization. Besides the confirmation of the typical fluorescence-emission signature of melanin and L-tyrosine, we provide additional insights on both emission and excitation spectra recorded during melanization. On these bases, we performed a subsequent characterization on the aqueous extracts from two different melanin-containing biological substrates, namely hairs from a domestic black cat and eggs from the Asian tiger mosquito. The results from the mild extraction procedure, purposely applied to obtain only the soluble components, combined with fluorescence spectral analysis are expected to promote further investigation of the melanization processes, particularly in insects.

Automated summary

Melanin is a natural pigment present in various organisms and is involved in processes such as immunity and antioxidant protection. This study characterized how melanin and its precursors respond to light irradiation through changes in fluorescence emission. The findings provide new insights into melanin production and its intermediates, and demonstrate the applications of these experimental procedures in investigating melanization processes in biological substrates, such as black cat hairs and mosquito eggs.