☆ 4.7 Article

HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography

COMMUNICATIONS BIOLOGY (2023)

Related references

Note: Only part of the references are listed.

Article Biochemical Research Methods

ELI trifocal microscope: a precise system to prepare target cryo-lamellae for in situ cryo-ET study

Shuoguo Li et al.

Summary: This study presents a precise cryogenic fabrication system, ELI-TriScope, which accurately and efficiently prepares target cryo-lamellae for cryo-electron tomography. It enables the discovery of new in situ structural features of cellular macromolecular machines.

NATURE METHODS (2023)

Add to Collection

Article Biochemical Research Methods

Integrated multimodality microscope for accurate and efficient target-guided cryo-lamellae preparation

Weixing Li et al.

Summary: Cryo-electron tomography is a revolutionary technique for resolving the structure of subcellular organelles and macromolecular complexes in their cellular context. However, the sample preparation step hampers its application. In this study, cryo-CLIEM technique was developed, which integrates three-dimensional confocal imaging with focused ion beam-scanning electron microscopy for efficient preparation of lamellae containing target structures. This approach has been shown to be generally applicable and will contribute to more applications of cryo-electron tomography.

NATURE METHODS (2023)

Add to Collection

Article Biotechnology & Applied Microbiology

Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy

Weisong Zhao et al.

Summary: Sparse structured illumination microscopy (Sparse-SIM) achieves nearly twofold resolution enhancement by utilizing sparse deconvolution algorithm, enabling the resolution of intricate biological structures such as small fusion pores, nuclear pores, and relative movements of inner and outer mitochondrial membranes.

NATURE BIOTECHNOLOGY (2022)

Add to Collection

Article Multidisciplinary Sciences

Structures from intact myofibrils reveal mechanism of thin filament regulation through nebulin

Zhexin Wang et al.

Summary: This study reveals the structure of nebulin bound to thin filaments and demonstrates its stabilizing role. It also highlights the different interactions of nebulin with actin and myosin, as well as its interaction with a troponin T linker, explaining its regulatory function.

SCIENCE (2022)

Add to Collection

Article Biochemical Research Methods

Isotropic super-resolution light-sheet microscopy of dynamic intracellular structures at subsecond timescales

Yuxuan Zhao et al.

Summary: Long-term visualization of dynamic interactions between intracellular structures in live cells is critical for understanding cellular functions. This study presents a novel approach that combines deep-learning super-resolution strategy and double-ring-modulated selective plane illumination microscopy design to achieve fast and high-resolution imaging of organelle interactions and dynamics in live cells.

NATURE METHODS (2022)

Add to Collection

Review Biochemistry & Molecular Biology

Drug discovery in the era of cryo-electron microscopy

Michael J. Robertson et al.

Summary: Structure-based drug discovery (SBDD) is an important approach for designing and optimizing new therapeutic agents. Cryo-electron microscopy (cryoEM) has become an exceptional tool for SBDD, providing a wealth of new structural information. In comparison to crystallization-based methods, cryoEM allows for the study of a wider range of systems and provides different forms of information, including heterogeneity and dynamics. Current and future developments in cryoEM aim to achieve the resolution and throughput necessary for it to have a widespread impact on the SBDD pipeline.

TRENDS IN BIOCHEMICAL SCIENCES (2022)

Add to Collection

Article Biology

A cryogenic, coincident fluorescence, electron, and ion beam microscope

Daan B. Boltje et al.

Summary: Cryo-ET combined with subtomogram averaging is a powerful technique for visualizing and determining the structure of macromolecular complexes. However, standard cryo-FIB fabrication used for sample preparation is blind to the precise location of structures or proteins of interest. In this study, a fluorescence microscopy-guided FIB fabrication method was developed to address this issue. By retrofitting a cryogenic microcooler and a fluorescence microscope onto an existing FIB scanning electron microscope, precise targeting at every milling step in the lamella fabrication process was achieved, resulting in improved success rate and throughput for high-resolution imaging.

ELIFE (2022)

Add to Collection

Article Multidisciplinary Sciences

Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility

Sudarshan Gadadhar et al.

Summary: This study demonstrates the importance of tubulin glycylation for controlled flagellar beating, directional sperm swimming, and male fertility by utilizing a mouse model lacking this modification. The lack of glycylation led to abnormal dynein arm conformations in sperm axonemes, resulting in subfertility in male mice due to aberrant beat patterns of sperm flagella.

SCIENCE (2021)

Add to Collection

Review Biochemistry & Molecular Biology

Molecular and cellular dynamics of the 26S proteasome

Eri Sakata et al.

Summary: The ubiquitin-proteasome system in eukaryotic cells removes dysfunctional or unneeded proteins, with the 26S proteasome being a multisubunit complex consisting of a 20S core particle and one or two regulatory particles. Advances in single-particle cryo-electron microscopy have provided atomic resolution models of the proteasome, contributing to a better understanding of its dynamics. Cryo-electron tomography allows for structural studies in situ, offering molecular resolution images of macromolecules within preserved cellular environments.

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS (2021)

Add to Collection

Article Biochemical Research Methods

Molecular-scale axial localization by repetitive optical selective exposure

Lusheng Gu et al.

Summary: The ROSE-Z method achieves super-resolution imaging with a <2 nm axial localization precision using an asymmetric optical scheme and approximately 3,000 photons. This method demonstrates nanoscale three-dimensional and two-color imaging, enhancing the investigation of cellular nanostructures.

NATURE METHODS (2021)

Add to Collection

Review Biophysics

Cryogenic superresolution correlative light and electron microscopy on the frontier of subcellular imaging

Buyun Tian et al.

Summary: Cryo-CLEM combines the advantages of electron microscopy and fluorescence microscopy to observe cellular components and their context in near-native state. This emerging imaging technique is expected to have wide applications in ultrastructural studies.

BIOPHYSICAL REVIEWS (2021)

Add to Collection

Article Biochemistry & Molecular Biology

VHUT-cryo-FIB, a method to fabricate frozen hydrated lamellae from tissue specimens for in situ cryo-electron tomography

Jianguo Zhang et al.

Summary: This study reports an integrated workflow, VHUT-cryo-FIB, for efficiently preparing frozen hydrated tissue lamella for cryo-electron tomography studies. The workflow was validated by obtaining in situ structures of ribosomes from spinach and liver samples.

JOURNAL OF STRUCTURAL BIOLOGY (2021)

Add to Collection

Article Biochemistry & Molecular Biology

CorRelator: Interactive software for real-time high precision cryo-correlative light and electron microscopy

Jie E. Yang et al.

Summary: Cryo-correlative light and electron microscopy (CLEM) combines the advantages of fluorescence light microscopy (FLM) and cryo-electron microscopy (cryo-EM) for high-resolution ultrastructure analysis of biological samples. Challenges lie in implementing correlation workflows and software tools on different microscope platforms to support automated cryo-EM data acquisition. CorRelator is an open-source desktop application that bridges between cryo-FLM and real-time cryo-EM/ET automated data collection, providing flexible and high accuracy correlation for cryo-CLEM workflows.

JOURNAL OF STRUCTURAL BIOLOGY (2021)

Add to Collection

Article Multidisciplinary Sciences

Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells

David P. Hoffman et al.

SCIENCE (2020)

Add to Collection

Review Biochemistry & Molecular Biology

Three-dimensional organization of the cytoskeleton: A cryo-electron tomography perspective

Saikat Chakraborty et al.

PROTEIN SCIENCE (2020)

Add to Collection

Article Optics

Deep learning-enhanced fluorescence microscopy via degeneration decoupling

Jiahao Liu et al.

OPTICS EXPRESS (2020)

Add to Collection

Article Biochemistry & Molecular Biology

3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway

Ilias Kounatidis et al.

CELL (2020)

Add to Collection

Article Biochemistry & Molecular Biology

Cryoelectron Tomography Reveals Nanoscale Organization of the Cytoskeleton and Its Relation to Microtubule Curvature Inside Cells

Saikat Chakraborty et al.

STRUCTURE (2020)

Add to Collection

Article Biochemistry & Molecular Biology

Multi-scale 3D Cryo-Correlative Microscopy for Vitrified Cells

Gong-Her Wu et al.

STRUCTURE (2020)

Add to Collection

Review Urology & Nephrology

Cellular signalling by primary cilia in development, organ function and disease

Zeinab Anvarian et al.

NATURE REVIEWS NEPHROLOGY (2019)

Add to Collection

Article Chemistry, Multidisciplinary

Increasing Resolution in Live Cell Microscopy by Structured Illumination (SIM)

Verena Richter et al.

APPLIED SCIENCES-BASEL (2019)

Add to Collection

Article Biology

PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

Sergey Gorelick et al.

ELIFE (2019)

Add to Collection

Article Biochemical Research Methods

Molecular resolution imaging by repetitive optical selective exposure

Lusheng Gu et al.

NATURE METHODS (2019)

Add to Collection

Article Cell Biology

Tricalbin-Mediated Contact Sites Control ER Curvature to Maintain Plasma Membrane Integrity

Javier Collado et al.

DEVELOPMENTAL CELL (2019)

Add to Collection

Review Cell Biology

Super-resolution microscopy demystified

Lothar Schermelleh et al.

NATURE CELL BIOLOGY (2019)

Add to Collection

Article Multidisciplinary Sciences

AutoCLEM: An Automated Workflow for Correlative Live-Cell Fluorescence Microscopy and Cryo-Electron Tomography

Xiaofeng Fu et al.

SCIENTIFIC REPORTS (2019)

Add to Collection

Review Physics, Multidisciplinary

Orienting the future of bio-macromolecular electron microscopy

Fei Sun

CHINESE PHYSICS B (2018)

Add to Collection

Article Biochemistry & Molecular Biology

High-vacuum optical platform for cryo-CLEM (HOPE): A new solution for non-integrated multiscale correlative light and electron microscopy

Shuoguo Li et al.

JOURNAL OF STRUCTURAL BIOLOGY (2018)

Add to Collection

Article Biochemistry & Molecular Biology

Visualizing Intracellular Organelle and Cytoskeletal Interactions at Nanoscale Resolution on Millisecond Timescales

Yuting Guo et al.

CELL (2018)

Add to Collection

Article Biochemical Research Methods

Correlated fluorescence microscopy and cryo-electron tomography of virus-infected or transfected mammalian cells

Cheri M. Hampton et al.

NATURE PROTOCOLS (2017)

Add to Collection

Article Biochemical Research Methods

Convolutional neural networks for automated annotation of cellular cryo-electron tomograms

Muyuan Chen et al.

NATURE METHODS (2017)

Add to Collection

Article Biophysics

Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy

Jan Arnold et al.

BIOPHYSICAL JOURNAL (2016)

Add to Collection

Article Biochemistry & Molecular Biology

ICON: 3D reconstruction with 'missing-information' restoration in biological electron tomography

Yuchen Deng et al.

JOURNAL OF STRUCTURAL BIOLOGY (2016)

Add to Collection

Article Biochemistry & Molecular Biology

An improved cryo-FIB method for fabrication of frozen hydrated lamella

Jianguo Zhang et al.

JOURNAL OF STRUCTURAL BIOLOGY (2016)

Add to Collection

Review Cell Biology

Cryo-Electron Tomography: Can it Reveal the Molecular Sociology of Cells in Atomic Detail?

Martin Beck et al.

TRENDS IN CELL BIOLOGY (2016)

Add to Collection

Review Biochemistry & Molecular Biology

Advances in electron microscopy: A qualitative view of instrumentation development for macromolecular imaging and tomography

Rasmus R. Schroeder

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2015)

Add to Collection

Review Biochemical Research Methods

Correlated light and electron microscopy: ultrastructure lights up!

Pascal de Boer et al.

NATURE METHODS (2015)

Add to Collection

Article Multidisciplinary Sciences

Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics

Dong Li et al.

SCIENCE (2015)

Add to Collection

Article Multidisciplinary Sciences

SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy

Graeme Ball et al.

SCIENTIFIC REPORTS (2015)

Add to Collection

Article Multidisciplinary Sciences

Three-dimensional super-resolution protein localization correlated with vitrified cellular context

Bei Liu et al.

SCIENTIFIC REPORTS (2015)

Add to Collection

Article Microscopy

Correlated cryo-fluorescence and cryo-electron microscopy with high spatial precision and improved sensitivity

Martin Schorb et al.

ULTRAMICROSCOPY (2014)

Add to Collection

Article Microscopy

High-precision correlative fluorescence and electron cryo microscopy using two independent alignment markers

Pascale Schellenberger et al.

ULTRAMICROSCOPY (2014)

Add to Collection

Article Biochemical Research Methods

Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton

Ke Xu et al.

NATURE METHODS (2012)

Add to Collection

Article Multidisciplinary Sciences

Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination

Reto Fiolka et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

Add to Collection

Article Biochemistry & Molecular Biology

Three-Dimensional Visualization of Gammaherpesvirus Life Cycle in Host Cells by Electron Tomography

Li Peng et al.

STRUCTURE (2010)

Add to Collection

Article Biophysics

Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination

Mats G. L. Gustafsson et al.

BIOPHYSICAL JOURNAL (2008)

Add to Collection

Article Biochemical Research Methods

Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics

Hari Shroff et al.

NATURE METHODS (2008)

Add to Collection

Article Multidisciplinary Sciences

Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell

Birka Hein et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)

Add to Collection

Article Biochemistry & Molecular Biology

Correlative microscopy: Bridging the gap between fluorescence light microscopy and cryo-electron tomography

Anna Sartori et al.

JOURNAL OF STRUCTURAL BIOLOGY (2007)

Add to Collection

Article Biochemistry & Molecular Biology

Automated electron microscope tomography using robust prediction of specimen movements

DN Mastronarde

JOURNAL OF STRUCTURAL BIOLOGY (2005)

Add to Collection

Article Chemistry, Multidisciplinary

UCSF chimera - A visualization system for exploratory research and analysis

EF Pettersen et al.

JOURNAL OF COMPUTATIONAL CHEMISTRY (2004)

Add to Collection

Review Virology

Herpesvirus assembly and egress

TC Mettenleiter

JOURNAL OF VIROLOGY (2002)

Add to Collection

© Peeref 2019-2024. All rights reserved.