Spironolactone as a Potential New Treatment to Prevent Arrhythmias in Arrhythmogenic Cardiomyopathy Cell Model

Arrhythmogenic cardiomyopathy (ACM) is a rare genetic disease associated with ventricular arrhythmias in patients. The occurrence of these arrhythmias is due to direct electrophysiological remodeling of the cardiomyocytes, namely a reduction in the action potential duration (APD) and a disturbance of Ca2+ homeostasis. Interestingly, spironolactone (SP), a mineralocorticoid receptor antagonist, is known to block K+ channels and may reduce arrhythmias. Here, we assess the direct effect of SP and its metabolite canrenoic acid (CA) in cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CMs) of a patient bearing a missense mutation (c.394C>T) in the DSC2 gene coding for desmocollin 2 and for the amino acid replacement of arginine by cysteine at position 132 (R132C). SP and CA corrected the APD in the muted cells (vs. the control) in linking to a normalization of the hERG and KCNQ1 K+ channel currents. In addition, SP and CA had a direct cellular effect on Ca2+ homeostasis. They reduced the amplitude and aberrant Ca2+ events. In conclusion, we show the direct beneficial effects of SP on the AP and Ca2+ homeostasis of DSC2-specific hiPSC-CMs. These results provide a rationale for a new therapeutical approach to tackle mechanical and electrical burdens in patients suffering from ACM.

Automated summary

Arrhythmogenic cardiomyopathy (ACM) is a rare genetic disease that leads to ventricular arrhythmias due to electrophysiological remodeling of cardiomyocytes. Spironolactone (SP), a mineralocorticoid receptor antagonist, has been found to reduce arrhythmias by blocking K+ channels. In this study, SP and its metabolite canrenoic acid (CA) were shown to correct abnormal action potential duration (APD) and Ca2+ homeostasis in cardiomyocytes derived from a patient with a specific mutation in the DSC2 gene. These findings provide a potential therapeutic approach for treating ACM patients by targeting mechanical and electrical burdens.