Glucose, Insulin and Oxygen Modulate Expression of Serotonin-Regulating Genes in Human First-Trimester Trophoblast Cell Line ACH-3P

Serotonin signaling plays an important role in regulating development and functions of the placenta. We hypothesized that metabolic disturbances associated with maternal obesity and/or gestational diabetes mellitus (GDM) affect placental serotonin homeostasis. Therefore, we examined the effects of high glucose (25 mM) and insulin (10 nM)-two hallmarks of maternal obesity and GDM-on mRNA expression of key regulators of serotonin homeostasis, including serotonin transporter (SERT), tryptophan hydroxylase 1 (TPH1), and monoamine oxidase A (MAOA), in the first-trimester trophoblast cell line ACH-3P, focusing on oxygen levels characteristic of early human placental development. Glucose downregulated expression of SERT and MAOA independently of oxygen level and upregulated expression of TPH1 at 6.5% oxygen but not at 2.5% oxygen. Compared to 6.5% oxygen, 2.5% oxygen upregulated SERT and downregulated TPH1 expression, with no effect on MAOA expression. Insulin upregulated SERT only at 2.5% oxygen but had no effect on TPH1 and MAOA expression. These results suggest that maternal metabolic alterations in early pregnancy may be a driving force for changes in placental serotonin homeostasis.

Automated summary

Serotonin signaling is important for regulating placental development and functions. Maternal metabolic disturbances associated with obesity and gestational diabetes may affect placental serotonin homeostasis. High glucose downregulates serotonin transporter (SERT) and monoamine oxidase A (MAOA), and upregulates tryptophan hydroxylase 1 (TPH1) at 6.5% oxygen. Low oxygen levels (2.5%) upregulate SERT and downregulate TPH1 expression. Insulin specifically upregulates SERT at 2.5% oxygen. These findings suggest that maternal metabolic alterations can impact placental serotonin homeostasis.