Journal
BIOACTIVE MATERIALS
Volume 24, Issue -, Pages 331-345Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2022.12.024
Keywords
Spinal cord injury; 3D printing; PCL oriented fibers; WAY-316606; Wnt; ?-catenin signaling
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This study constructs a composite biomimetic hydrogel composed of acellularized spinal cord matrix and gelatin-modified PEGDA hydrogel, loaded with WAY-316606, and reinforced by 3D printed polycaprolactone fibers. The composite hydrogel can fill spinal cord injury-induced cavities, promote neural tissue regeneration, and facilitate cell migration through its self-healing capability. Animal behavior studies demonstrate significant recovery of motor function in rats with spinal cord injury.
Spinal cord injury (SCI) is an overwhelming and incurable disabling condition, for which increasing forms of multifunctional biomaterials are being tested, but with limited progression. The promising material should be able to fill SCI-induced cavities and direct the growth of new neurons, with effective drug loading to improve the local micro-organism environment and promote neural tissue regeneration. In this study, a double crosslinked biomimetic composite hydrogel comprised of acellularized spinal cord matrix (ASCM) and gelatin-acrylated-beta-cyclodextrin-polyethene glycol diacrylate (designated G-CD-PEGDA) hydrogel, loaded with WAY-316606 to activate canonical Wnt/beta-catenin signaling, and reinforced by a bundle of three-dimensionally printed aligned polycaprolactone (PCL) microfibers, was constructed. The G-CD-PEGDA component endowed the composite hydrogel with a dynamic structure with a self-healing capability which enabled cell migration, while the ASCM component promoted neural cell affinity and proliferation. The diffusion of WAY-316606 could recruit endog-enous neural stem cells and improve neuronal differentiation. The aligned PCL microfibers guided neurite elongation in the longitudinal direction. Animal behavior studies further showed that the composite hydrogel could significantly recover the motor function of rats after SCI. This study provides a proficient approach to produce a multifunctional system with desirable physiological, chemical, and topographical cues for treating patients with SCI.
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