Diagnosis of Parkinson's Disease via the Metabolic Fingerprint in Saliva by Deep Learning

Parkinson's disease (PD) is the second cause of the neurodegenerative disorder, affecting over 6 million people worldwide. The World Health Organization estimated that population aging will cause global PD prevalence to double in the coming 30 years. Optimal management of PD shall start at diagnosis and requires both a timely and accurate method. Conventional PD diagnosis needs observations and clinical signs assessment, which are time-consuming and low-throughput. A lack of body fluid diagnostic biomarkers for PD has been a significant challenge, although substantial progress has been made in genetic and imaging marker development. Herein, a platform that noninvasively collects saliva metabolic fingerprinting (SMF) by nanoparticle-enhanced laser desorption-ionization mass spectrometry with high-reproducibility and high-throughput, using ultra-small sample volume (down to 10 nL), is developed. Further, excellent diagnostic performance is achieved with an area-under-the-curve of 0.8496 (95% CI: 0.7393-0.8625) by constructing deep learning model from 312 participants. In conclusion, an alternative solution is provided for the molecular diagnostics of PD with SMF and metabolic biomarker screening for therapeutic intervention.

Automated summary

Parkinson's disease (PD) is a neurodegenerative disorder that affects over 6 million people worldwide and is expected to double in prevalence due to population aging. Traditional PD diagnosis is time-consuming and low-throughput, lacking body fluid biomarkers. In this study, a noninvasive saliva metabolic fingerprinting (SMF) platform using nanoparticle-enhanced laser desorption-ionization mass spectrometry was developed, achieving excellent diagnostic performance with a deep learning model.