New Insight into the Genus Cladocroce (Porifera, Demospongiae) Based on Morphological and Molecular Data, with the Description of Two New Species

During scientific expeditions in Indonesia and Vietnam, several sponge specimens belonging to the genus Cladocroce were collected. The integration of morphological and molecular analyses, incorporating species delimitation models (ABGD, ASAP, and bPTP) and phylogenetic approaches using three molecular markers (COI, 28S, and 18S-ITS1-5.8S-ITS2-28S), allowed us to discriminate three congeneric species. Two of these species (C. burapha and C. pansinii sp. nov.) were supported by morphological and molecular data, whereas a third species (C. lamellata sp. nov.) was delimited by morphological data only. We formally describe two new species, C. pansinii sp. nov. and C. lamellata sp. nov. C. aculeata is a newly recorded species for Indonesia and the first documented finding after the original description. The re-examination of the type material of C. burapha, and indirectly the molecular approach, allowed us to confirm that C. burapha lives in sympatry with C. pansinii sp. nov. in Vietnam and with C. lamellata in Indonesia. Thanks to these findings, we relocated the paratype of C. burapha to the new species described here, i.e., C. pansinii sp. nov.

Automated summary

During scientific expeditions in Indonesia and Vietnam, sponge specimens of the genus Cladocroce were collected. By integrating morphological and molecular analyses, three congeneric species were identified using species delimitation models and phylogenetic approaches. Two new species, C. pansinii sp. nov. and C. lamellata sp. nov., were formally described. The re-examination of type material confirmed the coexistence of C. burapha with C. pansinii sp. nov. in Vietnam and with C. lamellata in Indonesia.