Development of multifunctional peptidomimetic poly(ester urethane)urea scaffolds loading with chlorogenic acid

Due to the limited bioactivities and negative host immune responses, it is extremely urgent to develop an alternative generation of synthetic polymers that are equipped with biological cues and matched biomechanical adaption for robust tissue regeneration. Considering overproduction of reactive oxygen species and infection during vascular replacement, herein, a kind of peptidomimetic lysine-based poly(ester urethane)urea (LPEUU) was synthesized and the bioactive fibrous scaffolds were developed by loading with chlorogenic acid (CGA) for the first time via electrospinning. It was found that the bioactivities of the LPEUU/CGA electrospun membranes were enhanced in facilitating vascular cell adhesion, spreading and proliferation performance. Especially, LPEUU loading with 1 wt. % or more CGA exhibited superior protective effects on human umbilical vein endothelial cells upon oxidative stress, and potent antibacterial activities against Escherichia coli and Staphylococcus aureus. Furthermore, in vivo subcutaneous implantation results showed that the LPEUU/CGA scaffold containing 1 wt. % CGA was infiltrated by more M2 macrophages, demonstrating as-expected regulation to host inflammatory response. The functional LPEUU scaffolds loading with CGA provided a great insight to the development of readily available vascular biomaterials.

Automated summary

In order to achieve robust tissue regeneration, a biocompatible and bioactive synthetic polymer, peptidomimetic lysine-based poly(ester urethane)urea (LPEUU), was successfully synthesized and electrospun into fibrous scaffolds for the first time. By loading chlorogenic acid (CGA), the bioactivity of LPEUU was enhanced, enabling better vascular cell adhesion, proliferation, and protection against oxidative stress and bacterial infection. In vivo experiments demonstrated that the LPEUU/CGA scaffold could regulate host inflammatory response. These functional LPEUU scaffolds loaded with CGA provide valuable insights for the development of easily accessible vascular biomaterials.