Comparative Evaluation of Bioefficiency and Photocatalytic Activity of Green Synthesized Bismuth Oxide Nanoparticles Using Three Different Leaf Extracts

Nanotechnology has emerged as a promising method for wastewater recycling. In this line, the current study emphasizes the leaf-extract-mediated biosynthesis of bismuth oxide nanostructures (Bi-ONPs) using three different plants, namely Coldenia procumbens Linn (Creeping Coldenia), Citrus limon (Lemon), and Murraya koenigii (Curry) through a greener approach and evaluates their biological properties as well as photocatalytic performance for the first time. As-synthesized BiONPs were physiochemically characterized using UV- visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and high-resolution transmission electron microscopy (HRTEM) with energy dispersive X-ray analysis (EDAX). Using the well diffusion method, research on the antibacterial efficiency of BiONPs against human pathogenic Gram-positive bacteria, such as Staphylococcus aureus and Enterococcus faecalis, and Gram-negative bacteria, including Escherichia coli and Klebsiella pneumonia, revealed that Gram-negative bacteria exhibited relatively strong activity. The larvicidal activity assessed against Aedes aegypti and Aedes albopictus mosquito larvae reveals promising larvicidal activity with a minimal dosage of BiONPs with LC50 values of 5.53 and 19.24 ppm, respectively, after 24 h of exposure. The excellent photocatalytic activity of as-synthesized BiONPs was demonstrated through the photodegradation of malachite green (MG) and methylene blue (MB) dyes with respective degradation performance parameters of 70 and 90%. The biogenic synthetic approach reported here enables the scalable commercial synthesis of bismuth nanostructures for their widespread use in catalysis for wastewater treatment and environmental cleanup.

Automated summary

The current study explores the leaf-extract-mediated biosynthesis of bismuth oxide nanostructures (Bi-ONPs) using three different plants and evaluates their biological properties and photocatalytic performance. The as-synthesized Bi-ONPs exhibit antibacterial and larvicidal activity, as well as excellent photocatalytic activity for dye degradation. This biogenic synthetic approach enables the scalable commercial synthesis of bismuth nanostructures for wastewater treatment and environmental cleanup.