4.6 Article

Synthesis and Application of Two-Photon Active Fluorescent Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging

Related references

Note: Only part of the references are listed.
Article Materials Science, Multidisciplinary

Aggregation effects on the one- and two-photon excited fluorescence performance of regioisomeric anthraquinone-substituted perylenediimide

Liang Xu et al.

Summary: In this study, two regioisomers of perylene diimide (PDI) derivatives were designed and synthesized. It was found that the positions of the substituent anthraquinone groups had distinct influences on the molecular structures and fluorescence properties, which were further explored in cellular imaging tests using nanoparticles.

JOURNAL OF MATERIALS CHEMISTRY C (2023)

Review Biochemical Research Methods

PET/Fluorescence Imaging: An Overview of the Chemical Strategies to Build Dual Imaging Tools

Julen Ariztia et al.

Summary: Molecular imaging is a biomedical research discipline that allows observation and quantification of biomarkers and biological processes. Combining multimodality imaging techniques offers unprecedented tools for human health. In this review, we focus on the combination of positron emission tomography and fluorescence imaging and summarize the chemical synthesis strategies for dual imaging agents.

BIOCONJUGATE CHEMISTRY (2022)

Article Chemistry, Organic

Benzothiazole-Derived Sulfones and Sulfoxides as Reactive Templates for Biothiols and Sulfane Sulfurs

Shi Xu et al.

Summary: In this study, we investigated the reactions of benzothiazole sulfones and sulfoxides with reactive sulfur species. The reaction between thiols and benzothiazole sulfones yielded sulfinic and thiosulfonic acids, which were utilized to develop fluorescent sensors for hydrogen polysulfides. The reaction of thiols with benzothiazole sulfoxides resulted in sulfenic acids, which could undergo intramolecular cyclization and were employed to design thiol-triggered fluorescent sensors.

ORGANIC LETTERS (2022)

Article Chemistry, Multidisciplinary

A SLC35C2 Transporter-Targeting Fluorescent Probe for the Selective Detection of B Lymphocytes Identified by SLC-CRISPRi and Unbiased Fluorescence Library Screening

Min Gao et al.

Summary: The development of a live B-cell selective small molecule probe CDyB has been achieved through unbiased fluorescence library screening. The molecular target of CDyB is identified as the SLC35C2 transporter. The fluorescence of CDyB and the expression of SLC35C2 are positively correlated with B-cell maturation.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022)

Article Biochemical Research Methods

One-Step Conversion of NHS Esters to Reagents for Site-Directed Labeling of IgG Antibodies

Rikke A. Hansen et al.

Summary: Antibody conjugates are widely used in diagnostics, therapeutics, and molecular biology. However, the current method of using NHS esters to prepare these conjugates leads to heterogeneous products. This study presents the development of a reagent that can be activated with a chosen NHS ester and used for site-directed labeling of antibodies in a single step. The reagent demonstrates similar ease of use as NHS esters but with improved site-directed labeling.

BIOCONJUGATE CHEMISTRY (2022)

Review Chemistry, Physical

Design, photophysical properties, and applications of fluorene-based fluorophores in two-photon fluorescence bioimaging: A review

Janah Shaya et al.

Summary: Two-photon fluorescence microscopy (2PFM) is a powerful alternative to traditional one-photon microscopy, using two near-infrared photons to excite fluorescent dyes and improve imaging quality in biological samples. Fluorene dyes possess excellent properties for 2PFM probes, including enhanced solubility, photostability, and two-photon absorption cross-section.

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY C-PHOTOCHEMISTRY REVIEWS (2022)

Article Chemistry, Multidisciplinary

General Design Strategy to Precisely Control the Emission of Fluorophores via a Twisted Intramolecular Charge Transfer (TICT) Process

Kenjiro Hanaoka et al.

Summary: This study establishes a new molecular design strategy for developing activatable fluorescent probes by controlling the twisted intramolecular charge transfer process. The fluorescence off/on mechanism based on this strategy is successfully applied to the development of practical probes for HaloTag and SNAP-tag, demonstrating its generalizability to other chemical dyes.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2022)

Article Chemistry, Organic

Red-emitting rhodamine-based probe with large Stokes shift for ClO-detection

Qin Liang et al.

Summary: A fluorescent probe for hypochlorite detection was developed and demonstrated efficient imaging capabilities in living cells and zebrafish.

TETRAHEDRON (2022)

Article Information Science & Library Science

The Past, Present and Future of the ELKH Cloud

Mihaly Heder et al.

Summary: This review article provides a summary of the history of the Hungarian Scientific Cloud Infrastructure project and its impact on cloud-computing enabled science in Hungary.

INFORMACIOS TARSADALOM (2022)

Article Oncology

Trastuzumab upregulates programmed death ligand-1 expression through interaction with NK cells in gastric cancer

Kohei Yamashita et al.

Summary: The study found that trastuzumab can upregulate PD-L1 expression on gastric cancer cells through interaction with NK cells. These results suggest clinical implications in the assessment of the predictive significance of PD-L1 expression for PD-1 inhibitors.

BRITISH JOURNAL OF CANCER (2021)

Review Biochemical Research Methods

Bioorthogonal Chemistry and Its Applications

Robert E. Bird et al.

Summary: Bioorthogonal chemistry is a method using non-native functional groups to explore biology in living organisms. This review summarizes common reactions in bioorthogonal methods and the frequency of bioorthogonal applications on different types of biomolecules. The application of bioorthogonal chemistry in imaging, drug delivery, and protein surface attachment is discussed, highlighting its current limitations and potential for future productive use.

BIOCONJUGATE CHEMISTRY (2021)

Article Chemistry, Organic

Synthesis and Fluorescence Mechanism of the Aminoimidazolone Analogues of the Green Fluorescent Protein: Towards Advanced Dyes with Enhanced Stokes Shift, Quantum Yield and Two-Photon Absorption

Ervin Kovacs et al.

Summary: Novel small-molecular analogues of the green fluorescence protein (GFP) chromophore were synthesised to expand and improve this fluorophore family and deepen the understanding of their fluorescence mechanism. The introduction of specific substituents and manipulation of the molecular structure successfully enhanced fluorescence emission intensity and altered spectral properties.

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY (2021)

Review Biochemical Research Methods

Squaric Acid-Based Radiopharmaceuticals for Tumor Imaging and Therapy

Tilmann Grus et al.

Summary: Targeting vectors bound to chelators with squaramides as coupling units offer advantages over common coupling methods in radiopharmaceuticals. This review focuses on the use of squaric acid diesters (SADE) as linking agents for conjugating chelators to different targeting vectors like prostate-specific membrane antigen inhibitors (PSMAi) and monoclonal antibodies (mAbs). The unique properties of SADE allow for fast and simple conjugation while potentially improving the pharmacokinetic properties of radiopharmaceuticals.

BIOCONJUGATE CHEMISTRY (2021)

Article Biochemical Research Methods

Ratiometric Fluorescence Monitoring of Antibody-Guided Drug Delivery to Cancer Cells

Dvir Poplinger et al.

Summary: Ratiometric measurements using dual-dye molecular systems have improved detection sensitivity and quantification in various assays, but have not been used for antibody-guided targeted drug delivery (TDD). A novel dual-dye TDD system, CySs-Ab-Flu-Aza, was successfully demonstrated for ratiometric fluorescence monitoring of drug release in vitro, showing potential for future applications in vivo with a longer-wavelength switchable fluorophore for quantification of drug release.

BIOCONJUGATE CHEMISTRY (2021)

Article Chemistry, Organic

A Comprehensive Study of the Ca2+ Ion Binding of Fluorescently Labelled BAPTA Analogues

Attila Csomos et al.

Summary: The comprehensive study on novel alterations in the structure of BAPTA revealed significant changes in the characteristics of the compounds, such as the effect of linker's length, the number of oxygen atoms in the chain, and removal of one of the rings. The results showed that even without the second aromatic ring, the fluorescence could still be turned on, and successful sensing could be achieved by replacing the chain with a single oxygen atom.

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY (2021)

Article Chemistry, Multidisciplinary

Theoretical Design, Synthesis, and In Vitro Neurobiological Applications of a Highly Efficient Two-Photon Caged GABA Validated on an Epileptic Case

Balazs Chiovini et al.

Summary: This paper introduces a new cage-GABA compound, iDMPO-DNI-GABA, which is currently the only photoreagent for GABA uncaging without experimental compromises. Through systematic theoretical design and synthesis, the best reagent exhibits high two-photon efficiency within the 700-760 nm range, demonstrating excellent pharmacological properties suitable for complex epileptic studies.

ACS OMEGA (2021)

Article Chemistry, Multidisciplinary

Stress response decay with aging visualized using a dual-channel logic-based fluorescent probe

Jingye Tian et al.

Summary: Diagnosing aging for preventative intervention often relies on tracking aging biomarkers. A new strategy using the dual-channel fluorescent probe ROKS allows for precise assessment of stress response capacity in individuals over time, showing that live cell SRC decreases with senescence and is higher in young vs. aged Caenorhabditis elegans. This study offers a promising method for fluorescence-guided diagnosis of aging and evaluating the effectiveness of anti-aging drugs.

CHEMICAL SCIENCE (2021)

Article Chemistry, Multidisciplinary

Synthesis and characterization of new fluorescent boro-β-carboline dyes

Denes Szepesi Kovacs et al.

Summary: The first representatives of a new fluorescent boro-beta-carboline family were synthesized by inserting difluoroboranyl group into oxaza or diaza core, exhibiting good photophysical properties with fine Stokes-shifts. Quantum chemical computations suggested improved properties of diazaborinino-carbolines over oxazaborolo-carbolines. This chemotype was nominated as a new fluorophore for developing fluorescent probes.

RSC ADVANCES (2021)

Review Materials Science, Multidisciplinary

The design strategies and applications for organic multi-branched two-photon absorption chromophores with novel cores and branches: a recent review

Liang Xu et al.

Summary: This study summarizes the recent advances in organic multi-branched chromophores (OMBCs) in the field of two-photon absorption materials, including their basic molecular structures, absorption properties, structure-property relationships, and various applications.

JOURNAL OF MATERIALS CHEMISTRY C (2021)

Review Oncology

Antibody-Drug Conjugates: A Comprehensive Review

Puregmaa Khongorzul et al.

MOLECULAR CANCER RESEARCH (2020)

Article Chemistry, Multidisciplinary

Synthesis and properties of seminaphthorhodafluor red laser dyes

Wei Ming et al.

RESEARCH ON CHEMICAL INTERMEDIATES (2020)

Article Biochemistry & Molecular Biology

An optimal Click formulation strategy for antibody-drug conjugate synthesis

Erol C. Vatansever et al.

BIOORGANIC & MEDICINAL CHEMISTRY (2020)

Review Materials Science, Multidisciplinary

Recent progress in efficient organic two-photon dyes for fluorescence imaging and photodynamic therapy

Liang Xu et al.

JOURNAL OF MATERIALS CHEMISTRY C (2020)

Article Chemistry, Multidisciplinary

Cysteine specific bioconjugation with benzyl isothiocyanates

Laszlo Petri et al.

RSC ADVANCES (2020)

Article Chemistry, Organic

Altering Fundamental Trends in the Emission of Xanthene Dyes

Lei G. Wang et al.

JOURNAL OF ORGANIC CHEMISTRY (2019)

News Item Biotechnology & Applied Microbiology

Click chemistry targets antibody-drug conjugates for the clinic

Mark Peplow

NATURE BIOTECHNOLOGY (2019)

Article Spectroscopy

Synthesis and spectroscopic characterization of novel GFP chromophore analogues based on aminoimidazolone derivatives

Attila Jancso et al.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2019)

Review Chemistry, Multidisciplinary

Rhodols - synthesis, photophysical properties and applications as fluorescent probes

Yevgen M. Poronik et al.

CHEMICAL SOCIETY REVIEWS (2019)

Review Biochemistry & Molecular Biology

Amide Activation in Ground and Excited States

Ervin Kovacs et al.

MOLECULES (2018)

Article Chemistry, Multidisciplinary

Synthesis and fluorescent properties of boroisoquinolines, a new family of fluorophores

Denes Sovari et al.

RSC ADVANCES (2018)

Article Materials Science, Multidisciplinary

Recent progress on intramolecular charge-transfer compounds as photoelectric active materials

Liang Xu et al.

SCIENCE CHINA-MATERIALS (2017)

Review Oncology

Tumor-associated stromal cells as key contributors to the tumor microenvironment

Karen M. Bussard et al.

BREAST CANCER RESEARCH (2016)

Article Chemistry, Multidisciplinary

A ratiometric fluorescent molecular probe for visualization of mitochondrial temperature in living cells

Mitsumasa Homma et al.

CHEMICAL COMMUNICATIONS (2015)

Review Oncology

Antibody-based imaging strategies for cancer

Jason M. Warram et al.

CANCER AND METASTASIS REVIEWS (2014)

Review Radiology, Nuclear Medicine & Medical Imaging

Towards detecting the HER-2 receptor and metabolic changes induced by HER-2-targeted therapies using medical imaging

T. A. D. Smith

BRITISH JOURNAL OF RADIOLOGY (2010)

Article Chemistry, Multidisciplinary

A Palette of Fluorescent Probes with Varying Emission Colors for Imaging Hydrogen Peroxide Signaling in Living Cells

Bryan C. Dickinson et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)

Review Chemistry, Multidisciplinary

Synthesis and applications of Rhodamine derivatives as fluorescent probes

Mariana Beija et al.

CHEMICAL SOCIETY REVIEWS (2009)