4.7 Article

A Fast and Cost-Effective Genotyping Method for CRISPR-Cas9-Generated Mutant Rice Lines

Related references

Note: Only part of the references are listed.
Article Plant Sciences

ZAXINONE SYNTHASE 2 regulates growth and arbuscular mycorrhizal symbiosis in rice

Abdugaffor Ablazov et al.

Summary: OsZAS2 is an enzyme involved in the synthesis of zaxinone in rice plants, which is required for proper growth, mycorrhization, and plant hormone homeostasis.

PLANT PHYSIOLOGY (2023)

Article Genetics & Heredity

Inference of CRISPR Edits from Sanger Trace Data

David Conant et al.

Summary: ICE is a tool for analyzing CRISPR edits using Sanger data, providing a fast, accurate, and affordable way to assess the genotype after editing. It proposes potential editing outcomes and determines the data-supported results through regression analysis. ICE can analyze CRISPR experiments within days after transfection and produces accurate estimates of editing outcomes in various benchmarks and existing tools.

CRISPR JOURNAL (2022)

Article Plant Sciences

Zaxinone synthase controls arbuscular mycorrhizal colonization level in rice

Cristina Votta et al.

Summary: OsZAS and exogenous zaxinone have different effects on mycorrhization, with OsZAS being involved in the regulation network through influencing strigolactone production.

PLANT JOURNAL (2022)

Article Plant Sciences

Accurate Direct PCR With Arabidopsis and Rice

Peter G Lynagh et al.

PLANT AND CELL PHYSIOLOGY (2022)

Article Genetics & Heredity

Deconvolution of Complex DNA Repair (DECODR): Establishing a Novel Deconvolution Algorithm for Comprehensive Analysis of CRISPR-Edited Sanger sequencing Data

Kevin Bloh et al.

Summary: DECODR is a comprehensive tool developed to detect a wide range of indels formed during CRISPR experiments, accurately identifying and outputting the positions and identities of inserted and deleted bases. It is suitable for experiments utilizing various configurations of donor DNA sequences, CRISPR-Cas, and endogenous DNA repair pathways.

CRISPR JOURNAL (2021)

Article Biology

Multi-omics approaches explain the growth-promoting effect of the apocarotenoid growth regulator zaxinone in rice

Jian You Wang et al.

Summary: Zaxinone promotes rice growth by enhancing root sugar uptake and metabolism, as well as modulating cytokinin content. This compound acts as a global regulator of the transcriptome and metabolome, highlighting its potential in improving rice performance.

COMMUNICATIONS BIOLOGY (2021)

Article Plant Sciences

Development of methods for effective identification of CRISPR/Cas9-induced indels in rice

Sukumar Biswas et al.

PLANT CELL REPORTS (2019)

Article Multidisciplinary Sciences

The apocarotenoid metabolite zaxinone regulates growth and strigolactone biosynthesis in rice

Jian You Wang et al.

NATURE COMMUNICATIONS (2019)

Review Agronomy

CRISPR/Cas9: Development and Application in Rice Breeding

Fernando Matias Romero et al.

RICE SCIENCE (2019)

Article Chemistry, Analytical

A universal method for direct PCR amplification of plant tissues

Yuping Li et al.

ANALYTICAL METHODS (2017)

Review Biochemistry & Molecular Biology

Genome Editing with CRISPR-Cas9: Can It Get Any Better?

Maximilian Haeussler et al.

JOURNAL OF GENETICS AND GENOMICS (2016)

Article Multidisciplinary Sciences

CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing

Jonas Dehairs et al.

SCIENTIFIC REPORTS (2016)

Letter Biochemistry & Molecular Biology

DSDecode: A Web-Based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations

Weizhi Liu et al.

MOLECULAR PLANT (2015)

Article Multidisciplinary Sciences

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

Kabin Xie et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Review Medicine, Research & Experimental

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

Xiao-Hui Zhang et al.

MOLECULAR THERAPY-NUCLEIC ACIDS (2015)

Letter Cell Biology

Targeted mutagenesis in rice using CRISPR-Cas system

Jin Miao et al.

CELL RESEARCH (2013)

Article Biochemistry & Molecular Biology

A high-throughput, high-quality plant genomic DNA extraction protocol

H. Li et al.

GENETICS AND MOLECULAR RESEARCH (2013)

Letter Biotechnology & Applied Microbiology

Targeted genome modification of crop plants using a CRISPR-Cas system

Qiwei Shan et al.

NATURE BIOTECHNOLOGY (2013)

Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

Article Plant Sciences

A RAPID AND INEXPENSIVE METHOD FOR THE DIRECT PCR AMPLIFICATION OF DNA FROM PLANTS

Dirk U. Bellstedt et al.

AMERICAN JOURNAL OF BOTANY (2010)

Article Biochemical Research Methods

A Simple Way to Treat PCR Products Prior to Sequencing Using ExoSAP-IT®

Jonathan R. Bell

BIOTECHNIQUES (2008)

Article Biochemistry & Molecular Biology

Rice mutant resources for gene discovery

H Hirochika et al.

PLANT MOLECULAR BIOLOGY (2004)