Understanding the Geographic Patterns of Closely-Related Species of Paspalum (Poaceae) Using Distribution Modelling and Seed Germination Traits

The sexual species of the Dilatata complex (Paspalum dasypleurum, P. flavescens, P. plurinerve, P. vacarianum, and P. urvillei) are closely related phylogenetically and show allopatric distributions, except P. urvillei. These species show microhabitat similarities and differences in germination traits. We integrated species distribution models (SDMs) and seed germination assays to determine whether germination divergences explain their biogeographic pattern. We trained SDMs in South America using species' presence-absence data and environmental variables. Additionally, populations sampled from highly favourable areas in the SDMs of these species were grown together, and their seeds germinated at different temperatures and dormancy-breaking conditions. Differences among species in seed dormancy and germination niche breadth were tested, and linear regressions between seed dormancy and climatic variables were explored. SDMs correctly classified both the observed presences and absences. Spatial factors and anthropogenic activities were the main factors explaining these distributions. Both SDMs and germination analyses confirmed that the niche of P. urvillei was broader than the other species which showed restricted distributions, narrower germination niches, and high correlations between seed dormancy and precipitation regimes. Both approaches provided evidence about the generalist-specialist status of each species. Divergences in seed dormancy between the specialist species could explain these allopatric distributions.

Automated summary

The sexual species of the Dilatata complex have closely related phylogenetic relationships but show allopatric distributions, except for P. urvillei. These species exhibit microhabitat similarities and differences in germination traits. By combining species distribution models (SDMs) and seed germination assays, it was found that germination divergences may explain their biogeographic pattern. The broader niche of P. urvillei and its correlation with precipitation regimes suggest a generalist status, while the specialist species with narrower germination niches exhibit allopatric distributions, possibly due to divergences in seed dormancy.