Novel Arginine- and Proline-Rich Candidacidal Peptides Obtained through a Bioinformatic Approach

Antimicrobial resistance is a major public health concern worldwide. Albeit to a lesser extent than bacteria, fungi are also becoming increasingly resistant to antifungal drugs. Moreover, due to the small number of antifungal classes, therapy options are limited, complicating the clinical management of mycoses. In this view, antimicrobial peptides (AMPs) are a potential alternative to conventional drugs. Among these, Proline-rich antimicrobial peptides (PrAMPs), almost exclusively of animal origins, are of particular interest due to their peculiar mode of action. In this study, a search for new arginine- and proline-rich peptides from plants has been carried out with a bioinformatic approach by sequence alignment and antimicrobial prediction tools. Two peptide candidates were tested against planktonic cells and biofilms of Candida albicans and Candida glabrata strains, including resistant isolates. These peptides showed similar potent activity, with half-maximal effective concentration values in the micromolar range. In addition, some structural and functional features, revealing peculiar mechanistic behaviors, were investigated.

Automated summary

Antimicrobial resistance, including fungal resistance, is a global public health concern. This study aimed to search for potential alternatives to conventional drugs, focusing on proline-rich antimicrobial peptides (PrAMPs) from plants. Two candidates showed potent activity against Candida albicans and Candida glabrata strains, including resistant isolates, with unique mechanistic properties.