Journal
FOODS
Volume 12, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/foods12081701
Keywords
in vitro gastrointestinal digestion; antioxidant properties; carotenoids; postharvest processing
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This study examined the impact of LAB 75 fermentation on the properties of cowpea leaf smoothies. Fermentation led to a decrease in pH and an increase in total titratable acidity. The smoothies also experienced color changes, with VOP 1 showing the least change. The fermentation process improved the antioxidant capacity of the smoothies, attributed to an increase in phenolic compounds and carotenoids.
This study investigated the effects of Lactiplantibacillus plantarum 75 (LAB 75) fermentation at 37 degrees C for 48 h on the pH, total soluble solids (TSS), colour, total titratable acidity (TTA), carotenoids, and bioactivities of cowpea leaf smoothies from three cultivars (VOP 1, VOP 3, and VOP 4). Fermentation reduced the pH from 6.57 to 5.05 after 48 h. The TTA increased with the fermentation period, whilst the TSS reduced. Fermentation of the smoothies resulted in the least colour changes ( increment E) in VOP 1 after 48 h. Fermentation of cowpea smoothies (VOP 1, VOP 3, and VOP 4) improved the antioxidant capacity (FRAP, DPPH, and ABTS), which was attributed to the increase in total phenolic compounds and carotenoid constituents in all of the fermented cowpea smoothies. VOP 1 was further selected for analysis due to its high phenolic content and antioxidant activity. The VOP 1 smoothie fermented for 24 h showed the lowest reduction in TPC (11%) and had the highest antioxidant (FRAP, DPPH, and ABTS) activity. Ltp. plantarum 75 was viable and survived the harsh conditions of the gastrointestinal tract, and, hence, could be used as a probiotic. VOP 1 intestinal digesta showed significantly higher glucose uptake relative to the undigested and the gastric digesta, while the gastric phase had higher levels of alpha-amylase and alpha-glucosidase compared to the undigested samples.
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