Journal
LIFE SCIENCES
Volume 144, Issue -, Pages 54-60Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2015.11.024
Keywords
Spinal trigeminal nucleus interpolaris; Long-term potentiation; Long-term depression; High-frequency stimulation; NMDA receptor-independent; mGluR1
Funding
- National Research Foundation of Korea (NRF) - Ministry of Education [2015R1D1A1A01059432]
- National Research Foundation of Korea [2015R1D1A1A01059432] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Aims: Patterns of synaptic activity determine synaptic strengthening or weakening that is typically represented as long-term potentiation (LTP) and long-term depression (LTD), respectively. In the present study, we aim to test whether a conditioning stimulation of the spinal trigeminal subnucleus caudalis (Vc) induces LTP at excitatory synapses in the subnucleus interpolaris (Vi) and to characterize the LTP. Main methods: Generally, a presynaptic high-frequency stimulation (HFS) protocol can induce LTP at excitatory synapses in the brain, including the spinal cord. Therefore, LTP in the Vi was induced by the HFS (3 tetani at 100 Hz) of Vc in the horizontal brainstem slices. By pretreating slices with antagonists for NMDA receptors, metabotropic glutamate receptor subtype 1 or 5 (mGluR1 or 5), GABA(A) receptors, glycine receptors and Ca2+ chelator, the LTP was characterized. Key findings: The HFS reliably but slowly induced LTP of excitatory synaptic transmission in the Vi. This LTP was not dependent on NMDA receptor activation; however, it did require the activation of mGluR1, but notmGluR5, and an intracellular Ca2+ rise. Interestingly, this LTP induction required inhibitory synaptic transmission mediated by GABA(A) and glycine receptors, and coincided with the slow development of LTD at GABAergic synapses. The GABAergic LTD was mediated by mGluR1 and the intracellular Ca2+ rise. Significance: These data suggest that the modulation of GABAergic synaptic transmission by conditioning synaptic activity contributes to the induction and expression of LTP at excitatory synapses in the Vi. (C) 2015 Elsevier Inc. All rights reserved.
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